Validation Data Gallery
Tested Applications
| Positive WB detected in | rat brain tissue, HeLa cells, mouse brain tissue |
| Positive IHC detected in | human liver cancer tissue, human colon tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 19 publications below |
| IHC | See 6 publications below |
| IF | See 5 publications below |
Product Information
20248-1-AP targets ROCK2-Specific(C-term) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | Rho-associated, coiled-coil containing protein kinase 2 |
| Calculated molecular weight | 161 kDa |
| Observed molecular weight | 164 kDa |
| GenBank accession number | NM_004850 |
| Gene Symbol | ROCK2 |
| Gene ID (NCBI) | 9475 |
| RRID | AB_10665551 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75116 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).This antibody is specific to ROCK2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ROCK2-Specific(C-term) antibody 20248-1-AP | Download protocol |
| IHC protocol for ROCK2-Specific(C-term) antibody 20248-1-AP | Download protocol |
| WB protocol for ROCK2-Specific(C-term) antibody 20248-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biomaterials Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis. | ||
Acta Biomater ROCK isoforms differentially modulate cancer cell motility by mechanosensing the substrate stiffness.
| ||
J Orthop Translat Augmentation of functional recovery via ROCK/PI3K/AKT pathway by Fasudil Hydrochloride in a rat sciatic nerve transection model | ||
Proc Natl Acad Sci U S A ROCK2 interacts with p22phox to phosphorylate p47phox and to control NADPH oxidase activation in human monocytes | ||
Int J Mol Med Inhibition of Rho kinase protects against colitis in mice by attenuating intestinal epithelial barrier dysfunction via MLC and the NF-κB pathway. |