Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HT-1080 using 21645-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21645-1-AP
WB result of ROCK2(middle) antibody (21645-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK2(middle) transfected HeLa cells.
WB analysis of HeLa using 21645-1-AP
WB result of ROCK2(middle) antibody (21645-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK2(middle) transfected HeLa cells.
WB analysis of mouse brain using 21645-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 21645-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 21645-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 21645-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21645-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 21645-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 21645-1-AP
IP result of anti-ROCK2(middle) (IP:21645-1-AP, 5ug; Detection:21645-1-AP 1:700) with HeLa cells lysate 1320ug.
IHC staining of human liver using 21645-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21645-1-AP (ROCK2(middle) antibody at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 21645-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21645-1-AP (ROCK2(middle) antibody at dilution of 1:200 (under 40x lens).
IHC staining of human liver using 21645-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 21645-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 21645-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ROCK2(middle) antibody (21645-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
IF Staining of HeLa using 21645-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ROCK2(middle) antibody (21645-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 750-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR006), CL594-phalloidin (red).

Featured Product
KD/KO Validated

ROCK2(middle) Polyclonal antibody

ROCK2(middle) Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 21645-1-AP

Publications(42)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ROCK2, EC:2.7.11.1, KIAA0619, p164 ROCK-2, Rho kinase 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (42)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HT-1080 cells, mouse brain tissue, HeLa cells, mouse lung tissue, MCF-7 cells, mouse liver tissue, L02 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 35 publications below
IHC	See 4 publications below
IF	See 7 publications below
IP	See 2 publications below

Product Information

21645-1-AP targets ROCK2(middle) in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag16330 Product name: Recombinant human ROCK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-180 aa of BC111801 Sequence: MEIDMTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERTLKQKVENLLLEAEKRCSLLDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQ 相同性解析による交差性が予測される生物種
Full Name	Rho-associated, coiled-coil containing protein kinase 2
Calculated molecular weight	77 kDa, 161 kDa
Observed molecular weight	161 kDa
GenBank accession number	BC111801
Gene Symbol	ROCK2
Gene ID (NCBI)	9475
RRID	AB_10858624
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75116
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).

Protocols

Product Specific Protocols
IF protocol for ROCK2(middle) antibody 21645-1-AP	Download protocol
IHC protocol for ROCK2(middle) antibody 21645-1-AP	Download protocol
IP protocol for ROCK2(middle) antibody 21645-1-AP	Download protocol
WB protocol for ROCK2(middle) antibody 21645-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cancer Hsa_circ_0125356 promotes gemcitabine resistance by modulating WNT canonical and non-canonical pathways via miR-582-5p/FGF9 axis in non-small cell lung cancer Authors - Xinyue Du View Article
human	WB	Drug Resist Updat Endothelial SMAD1-MCAM axis facilitates sunitinib resistance and progression of clear cell renal cell carcinoma via LAMB1-ITGB1 signaling. Authors - Hanzhong Zhang View Article
mouse	WB	Nat Commun In situ osteogenic activation of mesenchymal stem cells by the blood clot biomimetic mechanical microenvironment Authors - Wangxiao He View Article
mouse,human	WB	ACS Nano Graphene Oxide Induced Perturbation to Plasma Membrane and Cytoskeletal Meshwork Sensitize Cancer Cells to Chemotherapeutic Agents Authors - Jianqiang Zhu View Article
		Cell Rep Med Functional matrix vesicle replacement partially restores chemo-mechanical coupling in the aged bone niche. Authors - Liqiang Zhang View Article
		Cell Mol Biol Lett Targeting SLK protects against cerebral ischemia-reperfusion injury by regulating USP8-mediated HIF-1α stabilization and RhoA/ROCK activation. Authors - Yuetao Wen View Article

Validation Data Gallery

WB analysis of HT-1080 using 21645-1-AP

WB analysis of HeLa using 21645-1-AP

WB analysis of HeLa using 21645-1-AP

WB analysis of mouse brain using 21645-1-AP

WB analysis of mouse lung using 21645-1-AP

WB analysis of MCF-7 using 21645-1-AP

WB analysis of mouse liver using 21645-1-AP

WB analysis of HeLa using 21645-1-AP

WB analysis of L02 using 21645-1-AP

IP experiment of HeLa using 21645-1-AP

IHC staining of human liver using 21645-1-AP

IHC staining of human liver using 21645-1-AP

IHC staining of human liver using 21645-1-AP

IHC staining of human liver using 21645-1-AP

IF Staining of HepG2 using 21645-1-AP

IF Staining of HeLa using 21645-1-AP

ROCK2(middle) Polyclonal antibody

ROCK2(middle) Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Hsa_circ_0125356 promotes gemcitabine resistance by modulating WNT canonical and non-canonical pathways via miR-582-5p/FGF9 axis in non-small cell lung cancer

Endothelial SMAD1-MCAM axis facilitates sunitinib resistance and progression of clear cell renal cell carcinoma via LAMB1-ITGB1 signaling.

In situ osteogenic activation of mesenchymal stem cells by the blood clot biomimetic mechanical microenvironment

Graphene Oxide Induced Perturbation to Plasma Membrane and Cytoskeletal Meshwork Sensitize Cancer Cells to Chemotherapeutic Agents

Functional matrix vesicle replacement partially restores chemo-mechanical coupling in the aged bone niche.

Targeting SLK protects against cerebral ischemia-reperfusion injury by regulating USP8-mediated HIF-1α stabilization and RhoA/ROCK activation.

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