Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of HT-1080 using 21645-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21645-1-AP
WB result of ROCK2(middle) antibody (21645-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK2(middle) transfected HeLa cells.
WB analysis of mouse brain using 21645-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 21645-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 21645-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 21645-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21645-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 21645-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 21645-1-AP
WB result of ROCK2(middle) antibody (21645-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK2(middle) transfected HeLa cells.
IP experiment of HeLa using 21645-1-AP
IP result of anti-ROCK2(middle) (IP:21645-1-AP, 5ug; Detection:21645-1-AP 1:700) with HeLa cells lysate 1320ug.
IHC staining of human liver using 21645-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21645-1-AP (ROCK2(middle) antibody at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 21645-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21645-1-AP (ROCK2(middle) antibody at dilution of 1:200 (under 40x lens).
IHC staining of human liver using 21645-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 21645-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 21645-1-AP (ROCK2(middle) antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 21645-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ROCK2(middle) antibody (21645-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
IF Staining of HeLa using 21645-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ROCK2(middle) antibody (21645-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 750-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR006), CL594-phalloidin (red).

Featured Product
KD/KO Validated

ROCK2(middle) Polyclonal antibody

ROCK2(middle) Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 21645-1-AP

Publications(29)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ROCK2, EC:2.7.11.1, KIAA0619, p164 ROCK-2, Rho kinase 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (29)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HT-1080 cells, mouse brain tissue, HeLa cells, mouse lung tissue, MCF-7 cells, mouse liver tissue, L02 cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 27 publications below
IHC	See 4 publications below
IF	See 5 publications below
IP	See 2 publications below

Product Information

21645-1-AP targets ROCK2(middle) in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag16330 Product name: Recombinant human ROCK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-180 aa of BC111801 Sequence: MEIDMTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERTLKQKVENLLLEAEKRCSLLDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQ 相同性解析による交差性が予測される生物種
Full Name	Rho-associated, coiled-coil containing protein kinase 2
Calculated molecular weight	77 kDa, 161 kDa
Observed molecular weight	161 kDa
GenBank accession number	BC111801
Gene Symbol	ROCK2
Gene ID (NCBI)	9475
RRID	AB_10858624
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75116
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).

Protocols

Product Specific Protocols
IF protocol for ROCK2(middle) antibody 21645-1-AP	Download protocol
IHC protocol for ROCK2(middle) antibody 21645-1-AP	Download protocol
IP protocol for ROCK2(middle) antibody 21645-1-AP	Download protocol
WB protocol for ROCK2(middle) antibody 21645-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Int J Biol Sci TAGLN-RhoA/ROCK2-SLC2A3-mediated Mechano-metabolic Axis Promotes Skin Fibrosis Authors - Xinwei Cheng View Article
human	IHC	Oncotarget Phosphorylation of nucleophosmin at threonine 234/237 is associated with HCC metastasis. Authors - Rachel Hiu Ha Ching View Article
rat	WB,IF	Neurochem Res Pre-electroacupuncture Ameliorates Cerebral Ischemia-reperfusion Injury by Inhibiting Microglial RhoA/pyrin/GSDMD Signaling Pathway Authors - Hao Fang View Article
human	WB	Cells The Cytoskeleton Effectors Rho-Kinase (ROCK) and Mammalian Diaphanous-Related (mDia) Formin Have Dynamic Roles in Tumor Microtube Formation in Invasive Glioblastoma Cells. Authors - Kathryn N Becker View Article
rat	WB	Front Pharmacol Liraglutide Ameliorates Erectile Dysfunction via Regulating Oxidative Stress, the RhoA/ROCK Pathway and Autophagy in Diabetes Mellitus. Authors - Penghui Yuan View Article
mouse	WB	Mol Pharmacol Megakaryocytic Smad4 Regulates Platelet Function Through Syk and ROCK2 Expression. Authors - Yanhua Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HT-1080 using 21645-1-AP

WB analysis of HeLa using 21645-1-AP

WB analysis of mouse brain using 21645-1-AP

WB analysis of mouse lung using 21645-1-AP

WB analysis of MCF-7 using 21645-1-AP

WB analysis of mouse liver using 21645-1-AP

WB analysis of HeLa using 21645-1-AP

WB analysis of L02 using 21645-1-AP

WB analysis of HeLa using 21645-1-AP

IP experiment of HeLa using 21645-1-AP

IHC staining of human liver using 21645-1-AP

IHC staining of human liver using 21645-1-AP

IHC staining of human liver using 21645-1-AP

IHC staining of human liver using 21645-1-AP

IF Staining of HepG2 using 21645-1-AP

IF Staining of HeLa using 21645-1-AP

ROCK2(middle) Polyclonal antibody

ROCK2(middle) Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

TAGLN-RhoA/ROCK2-SLC2A3-mediated Mechano-metabolic Axis Promotes Skin Fibrosis

Phosphorylation of nucleophosmin at threonine 234/237 is associated with HCC metastasis.

Pre-electroacupuncture Ameliorates Cerebral Ischemia-reperfusion Injury by Inhibiting Microglial RhoA/pyrin/GSDMD Signaling Pathway

The Cytoskeleton Effectors Rho-Kinase (ROCK) and Mammalian Diaphanous-Related (mDia) Formin Have Dynamic Roles in Tumor Microtube Formation in Invasive Glioblastoma Cells.

Liraglutide Ameliorates Erectile Dysfunction via Regulating Oxidative Stress, the RhoA/ROCK Pathway and Autophagy in Diabetes Mellitus.

Megakaryocytic Smad4 Regulates Platelet Function Through Syk and ROCK2 Expression.