Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human gliomas tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
20220-1-AP targets ROBO3-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | roundabout, axon guidance receptor, homolog 3 (Drosophila) |
| Calculated molecular weight | 148 kDa |
| Observed molecular weight | 148 kDa |
| GenBank accession number | NM_022370 |
| Gene Symbol | ROBO3 |
| Gene ID (NCBI) | 64221 |
| RRID | AB_10732825 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96MS0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ROBO3 belongs to the immunoglobulin superfamily and ROBO family. ROBO3 is thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal cord development, it plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1. It is required for hindbrain axon midline crossing. Defects in ROBO3 are a cause of familial horizontal gaze palsy with progressive scoliosis (HGPPS). Patients show a medulla where motor and sensory projections appear uncrossed. The antibody is specific to ROBO3.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ROBO3-Specific antibody 20220-1-AP | Download protocol |
| IHC protocol for ROBO3-Specific antibody 20220-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Brain Res Bull CircSATB2 Modulates Fear Extinction Memory via Robo3-Driven Synaptic Plasticity |