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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNPS1 Polyclonal antibody (32844-1-AP)

    WB analysis using 32844-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32844-1-AP (RNPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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RNPS1 Polyclonal antibody

RNPS1 Polyclonal Antibody for WB, ELISA
Cat No. 32844-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

SR-related protein LDC2, RNPS 1, RNA-binding protein with serine-rich domain 1, LDC2, E5.1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, Raji cells, Ramos cells, fetal human brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32844-1-AP targets RNPS1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNPS1 fusion protein Ag37888 相同性解析による交差性が予測される生物種
Full Name RNA binding protein S1, serine-rich domain
Calculated molecular weight 34 kDa
Observed molecular weight45 kDa
GenBank accession numberBC001838
Gene Symbol RNPS1
Gene ID (NCBI) 10921
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15287
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The splicing process of mRNA must be highly precise to carry the correct genetic information to the cytoplasm as templates for translation. And this process involves more than 50 proteins in the splicing complex. RNPS1 is a versatile factor that regulates alternative splicing of a number of pre-mRNAs. It participates in both constitutive splicing and in distinctive modulation of alternative splicing in a substrate dependent manner, and involved in UPF2-dependent nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Its ser-53 phosphorylation site is important for splicing and translation stimulation activity in vitro.

Protocols

Product Specific Protocols
WB protocol for RNPS1 antibody 32844-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols