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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNPEP Polyclonal antibody (14764-1-AP)

    WB analysis using 14764-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14764-1-AP (RNPEP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RNPEP Polyclonal antibody (14764-1-AP)

    WB analysis of HepG2 using 14764-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14764-1-AP (RNPEP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using RNPEP Polyclonal antibody (14764-1-AP)

    WB analysis of A549 using 14764-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 14764-1-AP (RNPEP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A549 cells using RNPEP Polyclonal antibody (14764-1-AP)

    IP experiment of A549 using 14764-1-AP

    IP result of anti-RNPEP (IP:14764-1-AP, 4ug; Detection:14764-1-AP 1:500) with A549 cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RNPEP Polyclonal antibody (14764-1-AP)

    IHC staining of human lung cancer using 14764-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14764-1-AP (RNPEP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RNPEP Polyclonal antibody (14764-1-AP)

    IHC staining of human lung cancer using 14764-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14764-1-AP (RNPEP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO Validated

RNPEP Polyclonal antibody

RNPEP Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 14764-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

EC:3.4.11.6, AP-B, APB

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHL-60 cells, HepG2 cells, A549 cells, HeLa cells, K-562 cells, mouse liver tissue, rat liver tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14764-1-AP targets RNPEP in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6526

Product name: Recombinant human RNPEP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 300-650 aa of BC001064

Sequence: MENPCLTFVTPCLLAGDRSLADVIIHEISHSWFGNLVTNANWGEFWLNEGFTMYAQRRISTILFGAAYTCLEAATGRALLRQHMDITGEENPLNKLRVKIEPGVDPDDTYNETPYEKGFCFVSYLAHLVGDQDQFDSFLKAYVHEFKFRSILADDFLDFYLEYFPELKKKRVDIIPGFEFDRWLNTPGWPPYLPDLSPGDSLMKPAEELAQLWAAEELDMKAIEAVAISPWKTYQLVYFLDKILQKSPLPPGNVKKLGDTYPSISNARNAELRLRWGQIVLKNDHQEDFWKVKEFLHNQGKQKYTLPLYHAMMGGSEVAQTLAKETFASTASQLHSNVVNYVQQIVAPKGS

相同性解析による交差性が予測される生物種
Full Name arginyl aminopeptidase (aminopeptidase B)
Calculated molecular weight 72 kDa
Observed molecular weight 72 kDa
GenBank accession numberBC001064
Gene Symbol RNPEP
Gene ID (NCBI) 6051
RRIDAB_2254005
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H4A4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for RNPEP antibody 14764-1-APDownload protocol
IP protocol for RNPEP antibody 14764-1-APDownload protocol
WB protocol for RNPEP antibody 14764-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Autism

RNA sequencing and proteomics approaches reveal novel deficits in the cortex of Mecp2-deficient mice, a model for Rett syndrome.

Authors - Natasha L Pacheco
View Article
humanWB

Cancer Sci

Tumor exosomal RNPEP promotes lung metastasis of liver cancer via inducing cancer-associated fibroblast activation

Authors - Yuankun Chen
  • KD Validated
View Article
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