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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RNMTL1 Polyclonal antibody (14707-1-AP)

    WB analysis of HeLa using 14707-1-AP (same clone as 14707-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14707-1-AP (RNMTL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14707-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using RNMTL1 Polyclonal antibody (14707-1-AP)

    WB analysis of HEK-293 using 14707-1-AP (same clone as 14707-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14707-1-AP (RNMTL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14707-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using RNMTL1 Polyclonal antibody (14707-1-AP)

    IHC staining of human liver using 14707-1-AP (same clone as 14707-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14707-1-AP (RNMTL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14707-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using RNMTL1 Polyclonal antibody (14707-1-AP)

    IHC staining of human liver using 14707-1-AP (same clone as 14707-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14707-1-AP (RNMTL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14707-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RNMTL1 Polyclonal antibody (14707-1-AP)

    IF Staining of HeLa using 14707-1-AP (same clone as 14707-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RNMTL1 antibody (14707-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14707-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RNMTL1 Polyclonal antibody, PBS Only

RNMTL1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14707-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14707-1-PBS targets RNMTL1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag6399

Product name: Recombinant human RNMTL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-420 aa of BC050614

Sequence: MAALVRPSRFVVRPLLQVVQAWDLDARRWVRALRRSPVKVVFPSGEVVEQKRAPGKQPRKAPSEASAQEQREKQPLEESASRAPSTWEESGLRYDKAYPGDRRLSSVMTIVKSRPFREKQGKILLEGRRLISDALKAGAVPKMFFFSRLEYLKELPVDKLKGVSLIKVKFEDIKDWSDLVTPQGVMGIFAKPDHVKMTYPKTQLQHSLPLLLICDNLRDPGNLGTILRSAAGAGCSKVLLTKGCVDAWEPKVLRAGMGAHFRMPIINNLEWETVPNYLPPDTRVYVADNCGLYAQAEMSNKASDHGWVCDQRVMKFHKYEEEEDVETGASQDWLPHVEVQSYDSDWTEAPAAVVIGGETYGVSLESLQLAESTGGKRLLIPVVPGVDSLNSAMAASILLFEGKRQLRGRAEDLSRDRSYH

相同性解析による交差性が予測される生物種
Full Name RNA methyltransferase like 1
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC050614
Gene Symbol RNMTL1
Gene ID (NCBI) 55178
RRIDAB_2181563
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HC36
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

The RNA methyltransferase like 1 gene (RNMTL1) is one of thirteen newly discovered genes within a 116 Kb segment of the chromosome 17p13.3, which suffers from a high frequent loss of heterozygosity in human hepatocellular carcinoma. Its amino acid sequence was identified a high conserved region with methylases from various microorganisms, suggesting to have function to transfer methyl group to rRNA molecule [PMID:12296377].

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