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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human placenta tissue using RNGTT Recombinant antibody (83974-4-RR)

    IHC staining of human placenta using 83974-4-RR (same clone as 83974-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83974-4-RR (RNGTT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83974-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using RNGTT Recombinant antibody (83974-4-RR)

    IHC staining of human placenta using 83974-4-RR (same clone as 83974-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83974-4-RR (RNGTT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83974-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RNGTT Recombinant antibody (83974-4-RR)

    IF Staining of HepG2 using 83974-4-RR (same clone as 83974-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RNGTT antibody (83974-4-RR, Clone: 240927H2 ) at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83974-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using RNGTT Recombinant antibody (83974-4-RR)

    FC experiment of HepG2 using 83974-4-RR (same clone as 83974-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug RNGTT Recombinant antibody (83974-4-RR, Clone:240927H2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83974-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83974-4-PBS

    Affinity and Kinetic Characterization of 83974-4-PBS

    Biolayer interferometry (BLl) kinetic assay of 83974-4-PBS against Human RNGTT was performed. The affinity constant is 0.319 nM.

  • Affinity and Kinetic Characterization of 83974-4-RR

    Affinity and Kinetic Characterization of 83974-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83974-4-RR against Human RNGTT were performed. The affinity constant is below 1 pM.

RNGTT Recombinant monoclonal antibody, PBS Only

RNGTT Uni-rAb® Recombinant Antibody for IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 83974-4-PBS
Clone No.240927H2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

240927H2, CAP1A, GTase, GTP  RNA guanylyltransferase, GTP--RNA guanylyltransferase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83974-4-PBS targets RNGTT in IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag3105

Product name: Recombinant human RNGTT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 242-598 aa of BC019954

Sequence: TQVTTQPKLGEVQQKCHQFCGWEGSGFPGAQPVSMDKQNIKLLDLKPYKVSWKADGTRYMMLIDGTNEVFMIDRDNSVFHVSNLEFPFRKDLRMHLSNTLLDGEMIIDRVNGQAVPRYLIYDIIKFNSQPVGDCDFNVRLQCIEREIISPRHEKMKTGLIDKTQEPFSVRNKPFFDICTSRKLLEGNFAKEVSHEMDGLIFQPTGKYKPGRCDDILKWKPPSLNSVDFRLKITRMGGEGLLPQNVGLLYVGGYERPFAQIKVTKELKQYDNKIIECKFENNSWVFMRQRTDKSFPNAYNTAMAVCNSISNPVTKEMLFEFIDRCTAASQGQKRKHHLDPDTELMPPPPPKRPHPLT

相同性解析による交差性が予測される生物種
Full Name RNA guanylyltransferase and 5'-phosphatase
Calculated molecular weight 597 aa, 69 kDa
GenBank accession numberBC019954
Gene Symbol RNGTT
Gene ID (NCBI) 8732
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDO60942
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RNGTT(mRNA-capping enzyme) is also named as HCAP1, HCE, CAP1A. In the N-terminal section it belongs to the non-receptor class of the protein-tyrosine phosphatase family and in the C-terminal section it belongs to the eukaryotic GTase family. It is a bifunctional 597-amino acid protein with RNA triphosphatase and RNA guanylyltransferase activities(PMID:9545288). It has 4 isoforms produced by alternative splicing.

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