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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RNF8 Polyclonal antibody (14112-1-AP)

    WB analysis of HeLa using 14112-1-AP (same clone as 14112-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14112-1-AP (RNF8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RNF8 Polyclonal antibody (14112-1-AP)

    WB analysis using 14112-1-AP (same clone as 14112-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14112-1-AP (RNF8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14112-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using RNF8 Polyclonal antibody (14112-1-AP)

    WB analysis of HEK-293 using 14112-1-AP (same clone as 14112-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 14112-1-AP (RNF8 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14112-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using RNF8 Polyclonal antibody (14112-1-AP)

    IP experiment of HeLa using 14112-1-AP (same clone as 14112-1-PBS)

    IP result of anti-RNF8 (IP:14112-1-AP, 4ug; Detection:14112-1-AP 1:600) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 14112-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using RNF8 Polyclonal antibody (14112-1-AP)

    IHC staining of human testis using 14112-1-AP (same clone as 14112-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14112-1-AP (RNF8 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14112-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using RNF8 Polyclonal antibody (14112-1-AP)

    IHC staining of human testis using 14112-1-AP (same clone as 14112-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14112-1-AP (RNF8 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14112-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RNF8 Polyclonal antibody (14112-1-AP)

    IF Staining of HeLa using 14112-1-AP (same clone as 14112-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RNF8 antibody (14112-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 14112-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RNF8 Polyclonal antibody, PBS Only

RNF8 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14112-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14112-1-PBS targets RNF8 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag5292

Product name: Recombinant human RNF8 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 130-485 aa of BC007517

Sequence: ETIYPCLSPKNDQMIEKNKELRTKRKFSLDELAGPGAEGPSNLKSKINKVSCESGQPVKSQGKGEVASTPSDNLDPKLTALEPSKTTGAPIYPGFPKVTEVHHEQKASNSSASQRSLQMFKVTMSRILRLKIQMQEKHEAVMNVKKQTQKGNSKKVVQMEQELQDLQSQLCAEQAQQQARVEQLEKTFQEEEQHLQGLEIAQGEKDLKQQLAQALQEHWALMEELNRSKKDFEAIIQAKNKELEQTKEEKEKMQAQKEEVLSHMNDVLENELQCIICSEYFIEAVTLNCAHSFCSYCINEWMKRKIECPICRKDIKSKTYSLVLDNCINKMVNNLSSEVKERRIVLIRERKAKRLF

相同性解析による交差性が予測される生物種
Full Name ring finger protein 8
Calculated molecular weight 485 aa, 56 kDa
Observed molecular weight 56-60 kDa
GenBank accession numberBC007517
Gene Symbol RNF8
Gene ID (NCBI) 9025
RRIDAB_2878016
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO76064
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RNF8, also named as KIAA0646 or E3 ubiquitin-protein ligase RNF8, is a 485 amino acid protein, which contains one RING-type zinc finger and one FHA domain. RNF8 localizes in the nucleus and belongs to the RNF8 family. E2-E3 ubiquitin ligase complex is composed of the RNF8 homodimer and a E2 heterodimer of UBE2N and UBE2V2. E3 ubiquitin-protein ligase that plays a key role in DNA damage signaling via 2 distinct roles: by mediating the 'Lys-63'-linked ubiquitination of histones H2A and H2AX and promoting the recruitment of DNA repair proteins at double-strand breaks (DSBs) sites, and by catalyzing 'Lys-48'-linked ubiquitination to remove target proteins from DNA damage sites.

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