Validation Data Gallery
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Tested Applications
| Positive WB detected in | MDA-MB-231 cells, SH-SY5Y cells, mouse kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20105-1-AP targets RNF5 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13737 Product name: Recombinant human RNF5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-125 aa of BC004155 Sequence: MAAAEEEDGGPEGPNRERGGAGATFECNICLETAREAVVSVCGHLYCWPCLHQWLETRPERQECPVCKAGISREKVVPLYGRGSQKPQDPRLKTPPRPQGQRPAPESRGGFQPFGDTGGFHFSFG 相同性解析による交差性が予測される生物種 |
| Full Name | ring finger protein 5 |
| Calculated molecular weight | 180 aa, 20 kDa |
| Observed molecular weight | 15-50 kDa |
| GenBank accession number | BC004155 |
| Gene Symbol | RNF5 |
| Gene ID (NCBI) | 6048 |
| RRID | AB_3669323 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99942 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The RING (really interesting new gene) finger protein 5 (RNF5) is an ER-associated E3 ubiquitin ligase involved in ER-associated degradation (ERAD) for misfolded proteins.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF5 antibody 20105-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |