Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse ovary tissue, mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
88043-1-RR targets RNF41 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag11076 Product name: Recombinant human RNF41 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-317 aa of BC032637 Sequence: MGYDVTRFQGDVDEDLICPICSGVLEEPVQAPHCEHAFCNACITQWFSQQQTCPVDRSVVTVAHLRPVPRIMRNMLSKLQIACDNAVFGCSAVVRLDNLMSHLSDCEHNPKRPVTCEQGCGLEMPKDELPNHNCIKHLRSVVQQQQTRIAELEKTSAEHKHQLAEQKRDIQLLKAYMRAIRSVNPNLQNLEETIEYNEILEWVNSLQPARVTRWGGMISTPDAVLQAVIKRSLVESGCPASIVNELIENAHERSWPQGLATLETRQMNRRYYENYVAKRIPGKQAVVVMACENQHMGDDMVQEPGLVMIFAHGVEEI 相同性解析による交差性が予測される生物種 |
| Full Name | ring finger protein 41 |
| Calculated molecular weight | 317 aa, 36 kDa |
| Observed molecular weight | 35-40 kDa |
| GenBank accession number | BC032637 |
| Gene Symbol | RNF41 |
| Gene ID (NCBI) | 10193 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9H4P4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RNF41 acts as an E3 ubiquitin ligase to promote ErbB3 ubiquitination and degradation in cultured cells through its RING finger domain (PMID: 12411582). RNF41 acts also as a PRKN modifier that accelerates its degradation, resulting in a reduction of PRKN activity, influencing the balance of the intracellular redox state (PMID: 18541373).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF41 antibody 88043-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |