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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using RNF4 Polyclonal antibody (17810-1-AP)

    WB analysis of PC-3 using 17810-1-AP (same clone as 17810-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 17810-1-AP (RNF4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17810-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using RNF4 Polyclonal antibody (17810-1-AP)

    WB analysis of HepG2 using 17810-1-AP (same clone as 17810-1-PBS)

    WB result of RNF4 antibody (17810-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNF4 transfected HepG2 cells. This data was developed using the same antibody clone with 17810-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of PC-3 cells using RNF4 Polyclonal antibody (17810-1-AP)

    IP experiment of PC-3 using 17810-1-AP (same clone as 17810-1-PBS)

    IP result of anti-RNF4 (IP:17810-1-AP, 4ug; Detection:17810-1-AP 1:1500) with PC-3 cells lysate 1800 ug. This data was developed using the same antibody clone with 17810-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RNF4 Polyclonal antibody (17810-1-AP)

    IHC staining of mouse testis using 17810-1-AP (same clone as 17810-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17810-1-AP (RNF4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17810-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RNF4 Polyclonal antibody (17810-1-AP)

    IHC staining of mouse testis using 17810-1-AP (same clone as 17810-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17810-1-AP (RNF4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17810-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RNF4 Polyclonal antibody, PBS Only

RNF4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 17810-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

E3 ubiquitin ligase RNF4, E3 ubiquitin-protein ligase RNF4, EC:2.3.2.27, Protein SNURF, RES4 26

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17810-1-PBS targets RNF4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12115

Product name: Recombinant human RNF4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-190 aa of BC031935

Sequence: MSTRKRRGGAINSRQAQKRTREATSTPEISLEAEPIELVETAGDEIVDLTCESLEPVVVDLTHNDSVVIVDERRRPRRNARRLPQDHADSCVVSSDDEELSRDRDVYVTTHTPRNARDEGATGLRPSGTVSCPICMDGYSEIVQNGRLIVSTECGHVFCSQCLRDSLKNANTCPTCRKKINHKRYHPIYI

相同性解析による交差性が予測される生物種
Full Name ring finger protein 4
Calculated molecular weight 190 aa, 21 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC031935
Gene Symbol RNF4
Gene ID (NCBI) 6047
RRIDAB_2878443
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP78317
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RNF4 is a E3 ubiquitin-protein ligase that binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. It regulates the degradation of several proteins including PML and PEA3[PMID:18408734]. It also participates in chromosome alignment and spindle assembly, and regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. In addition, RNF4 can regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1 [PMID:20026589]. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation [PMID:11319220]. RNF4 exist as two isoforms. Isoform one is a 30 kDa protein and isoform two is a about 15 kDa protein. RNF4 binds polysumoylated chains and the molecular weight of RNF4 migrate in the experiment to 35 kDa, 47 kDa, 69 kDa as ubiquitinated protein.

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