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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using RNF38 Polyclonal antibody (25132-1-AP)

    WB analysis of HepG2 using 25132-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25132-1-AP (RNF38 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using RNF38 Polyclonal antibody (25132-1-AP)

    IHC staining of human endometrial cancer using 25132-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25132-1-AP (RNF38 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using RNF38 Polyclonal antibody (25132-1-AP)

    IHC staining of human endometrial cancer using 25132-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25132-1-AP (RNF38 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RNF38 Polyclonal antibody (25132-1-AP)

    IF Staining of HepG2 using 25132-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 25132-1-AP (RNF38 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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RNF38 Polyclonal antibody

RNF38 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25132-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ring finger protein 38, RNF38

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25132-1-AP targets RNF38 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF38 fusion protein Ag16054 相同性解析による交差性が予測される生物種
Full Name ring finger protein 38
Calculated molecular weight 515 aa, 58 kDa
Observed molecular weight 43-58 kDa
GenBank accession numberBC033786
Gene Symbol RNF38
Gene ID (NCBI) 152006
RRIDAB_2879914
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H0F5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RING finger protein 38 is a protein that in humans is encoded by the RNF38 gene.This gene encodes a protein with a coiled-coil motif and a RING-H2 motif (C3H2C2) at its carboxy-terminus. The RING motif is a zinc-binding domain found in a large set of proteins playing roles in diverse cellular processes including oncogenesis, development, signal transduction, and apoptosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. RNF38 is widely expressed with highest levels in testis. RNF38 exists various isoforms and molecular weight of isoforms are 36, 48, 52 and 58 kDa

Protocols

Product Specific Protocols
WB protocol for RNF38 antibody 25132-1-APDownload protocol
IHC protocol for RNF38 antibody 25132-1-APDownload protocol
IF protocol for RNF38 antibody 25132-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Cancer

Ring finger protein 38 promote non-small cell lung cancer progression by endowing cell EMT phenotype.

Authors - Dian Xiong
View Article