Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17471-1-AP targets RNF32 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11428 Product name: Recombinant human RNF32 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 4-221 aa of BC028120 Sequence: NKGHSSKKDNLAVNAVALQDHILHDLQLRNLSVADHSKTQVQKKENKSLKRDTKAIIDTGLKKTTQCPKLEDSEKEYVLDPKPPPLTLAQKLGLIGPPPPPLSSDEWEKVKQRSLLQGDSVQPCPICKEEFELRPQVLLSCSHVFHKACLQAFEKFTNKKTCPLCRKNQYQTRVIHDGARLFRIKCVTRIQAYWRGCVVRKWYRNLRKTVPPTDAKLR 相同性解析による交差性が予測される生物種 |
| Full Name | ring finger protein 32 |
| Calculated molecular weight | 362 aa, 42 kDa |
| Observed molecular weight | 42 kDa |
| GenBank accession number | BC028120 |
| Gene Symbol | RNF32 |
| Gene ID (NCBI) | 140545 |
| RRID | AB_3741919 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H0A6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ring Finger Protein 32 (RNF32) is a member of the RING finger protein family, characterized by a RING domain that functions as an E3 ubiquitin ligase. RNF32 is specifically expressed in testis and ovary. Studies indicate that RNF32 is expressed during spermatogenesis, most likely in spermatocytes and/or in spermatids, suggesting a possible role in sperm formation (PMID: 11890671, PMID: 41167191).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF32 antibody 17471-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |