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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNF31 / HOIP Recombinant monoclonal antibody (83137-4-RR)

    WB analysis using 83137-4-RR (same clone as 83137-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83137-4-RR (RNF31 / HOIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83137-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of PC-3 cells using RNF31 / HOIP Recombinant monoclonal antibody (83137-4-RR)

    FC experiment of PC-3 using 83137-4-RR (same clone as 83137-4-PBS)

    1x10^6 PC-3 cells were intracellularly stained with 0.2 ug RNF31 / HOIP Recombinant antibody (83137-4-RR, Clone:230446E9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83137-4-PBS in a different storage buffer formulation.

RNF31 / HOIP Recombinant monoclonal antibody, PBS Only

RNF31 / HOIP Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 83137-4-PBS
Clone No.230446E9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

HOIP, RNF31, 230446E9, E3 ubiquitin-protein ligase RNF31, EC:2.3.2.31

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83137-4-PBS targets RNF31 / HOIP in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34505

Product name: Recombinant human RNF31 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 459-603 aa of BC017376

Sequence: RDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPGGGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVSLINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKLTEEVPLGQSIPRRRK

相同性解析による交差性が予測される生物種
Full Name ring finger protein 31
Calculated molecular weight 1072 aa, 120 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC017376
Gene Symbol RNF31
Gene ID (NCBI) 55072
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ96EP0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.