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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNF31 / HOIP Polyclonal antibody (30797-1-AP)

    WB analysis using 30797-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30797-1-AP (RNF31 / HOIP antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RNF31 / HOIP Polyclonal antibody (30797-1-AP)

    WB analysis of HEK-293 using 30797-1-AP

    WB result of RNF31 / HOIP antibody (30797-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNF31 / HOIP transfected HEK-293 cells.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RNF31 / HOIP Polyclonal antibody (30797-1-AP)

    IF Staining of HepG2 using 30797-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNF31 / HOIP antibody (30797-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO Validated

RNF31 / HOIP Polyclonal antibody

RNF31 / HOIP Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30797-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

FLJ10111, FLJ23501, HOIL 1 interacting protein, HOIP, ring finger protein 31, RNF31, ZIBRA

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, Raji cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30797-1-AP targets RNF31 / HOIP in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag34505

Product name: Recombinant human RNF31 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 459-603 aa of BC017376

Sequence: RDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPGGGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVSLINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKLTEEVPLGQSIPRRRK

相同性解析による交差性が予測される生物種
Full Name ring finger protein 31
Calculated molecular weight 1072 aa, 120 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC017376
Gene Symbol RNF31
Gene ID (NCBI) 55072
RRIDAB_3086418
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96EP0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RNF31 / HOIP antibody 30797-1-APDownload protocol
IF protocol for RNF31 / HOIP antibody 30797-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols