Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293T cells |
| Positive IP detected in | A431 cells, HEK-293 cells |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
24988-1-AP targets RNF219 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21767 Product name: Recombinant human RNF219 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 271-416 aa of BC136764 Sequence: CQTALSADGKGSKGSEEDVVSKNQGDSARKQPGSSTSSSSHLAKPSSSRLCDTSSARQESTSKADLNCSKNKDLYQEQVEVMLDVTDTSMDTYLEREWGNKPSDCVPYKDEELYDLPAPCTPLSLSCLQLSTPENRESSVVQAGGS 相同性解析による交差性が予測される生物種 |
| Full Name | ring finger protein 219 |
| Calculated molecular weight | 726 aa, 81 kDa |
| Observed molecular weight | 80-85 kDa |
| GenBank accession number | BC136764 |
| Gene Symbol | RNF219 |
| Gene ID (NCBI) | 79596 |
| RRID | AB_2879832 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5W0B1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RNF219 antibody 24988-1-AP | Download protocol |
| IP protocol for RNF219 antibody 24988-1-AP | Download protocol |
| WB protocol for RNF219 antibody 24988-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
