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Validation Data Gallery

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  • Western Blot (WB) analysis of U2OS cells using RNF216 Polyclonal antibody (10185-1-AP)

    WB analysis of U2OS using 10185-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 10185-1-AP (RNF216 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SW480 cells using RNF216 Polyclonal antibody (10185-1-AP)

    WB analysis of SW480 using 10185-1-AP

    SW480 cells were subjected to SDS PAGE followed by western blot with 10185-1-AP (RNF216 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RNF216 Polyclonal antibody (10185-1-AP)

    WB analysis using 10185-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10185-1-AP (RNF216 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RNF216 Polyclonal antibody (10185-1-AP)

    WB analysis using 10185-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10185-1-AP (RNF216 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of SW480 cells using RNF216 Polyclonal antibody (10185-1-AP)

    IP experiment of SW480 using 10185-1-AP

    IP result of anti-RNF216 (IP:10185-1-AP, 4ug; Detection:10185-1-AP 1:1000) with SW480 cells lysate 1280 ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RNF216 Polyclonal antibody (10185-1-AP)

    IHC staining of mouse brain using 10185-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10185-1-AP (RNF216 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using RNF216 Polyclonal antibody (10185-1-AP)

    IHC staining of mouse heart using 10185-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10185-1-AP (RNF216 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RNF216 Polyclonal antibody (10185-1-AP)

    IF Staining of A431 using 10185-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using RNF216 antibody (10185-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

RNF216 Polyclonal antibody

RNF216 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10185-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

E3 ubiquitin-protein ligase RNF216, EC:2.3.2.27, RING finger protein 216, RING-type E3 ubiquitin transferase RNF216, Triad domain-containing protein 3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse heart tissue, U2OS cells, SW480 cells, mouse kidney tissue, rat heart tissue
Positive IP detected inSW480 cells
Positive IHC detected inmouse brain tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10185-1-AP targets RNF216 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0238

Product name: Recombinant human RNF216 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 73-276 aa of BC000787

Sequence: KRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKLELSCMEGSCTCSFPTSELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPHCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTS

相同性解析による交差性が予測される生物種
Full Name ring finger protein 216
Calculated molecular weight 48 kDa
Observed molecular weight57 kDa, 90-99 kDa, 106~130 kDa
GenBank accession numberBC000787
Gene Symbol RNF216
Gene ID (NCBI) 54476
RRIDAB_2180806
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NWF9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNF216/TRIAD3 is an RBR (RING-between-RING) E3 ligase that encodes for multiple isoforms that include TRIAD3A, TRIAD3B, TRIAD3C and TRIAD3D/E. RNF216 is a broadly expressed RBR E3 ligase, and loss-of-function mutations in RNF216 are linked to the neurode-generative conditions Gordon-Holmes syndrome (GHS) and Huntington-like disorders (PMID: 34998453, 35620441). RNF216 has three different molecular weights, 99 kDa, 106 kDa and 57 kDa, respectively.

Protocols

Product Specific Protocols
IF protocol for RNF216 antibody 10185-1-APDownload protocol
IHC protocol for RNF216 antibody 10185-1-APDownload protocol
IP protocol for RNF216 antibody 10185-1-APDownload protocol
WB protocol for RNF216 antibody 10185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Mol Med

Xanthatin synergizes with cisplatin to suppress homologous recombination through JAK2/STAT4/BARD1 axis in human NSCLC cells.

Authors - Jian Zhang
View Article
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