Validation Data Gallery
Tested Applications
| Positive IP detected in | Ramos cells |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32939-1-AP targets RNF187 in IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36665 Product name: Recombinant human RNF187 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 120-235 aa of BC012758 Sequence: EPRWRKALRGKENKGSVEIMRKDLNDARDLHGQAESAAAVWKGHVMDRRKKALTDYKKLRAFFVEEEEHFLQEAEKEEGLPEDELADPTERFRSLLQAVSELEKKHRNLGLSMLLQ 相同性解析による交差性が予測される生物種 |
| Full Name | ring finger protein 187 |
| Observed molecular weight | 29 kDa |
| GenBank accession number | BC012758 |
| Gene Symbol | RNF187 |
| Gene ID (NCBI) | 149603 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q5TA31 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RNF187 (Ring Finger Protein 187) is an E3 ubiquitin ligase with a molecular weight of approximately 28 kDa. It belongs to the RING finger protein family and participates in protein degradation and signal transduction regulation by catalyzing the ubiquitination of substrate proteins. In cancer research, RNF187 is upregulated in various malignant tumors, including melanoma and breast cancer, where it promotes tumor proliferation and invasion through ubiquitin-mediated degradation of tumor suppressors or regulation of cyclins. Additionally, RNF187 is involved in oxidative stress responses and DNA damage repair, playing a role in cell survival and maintenance of genomic stability. In research settings, anti-RNF187 antibodies are widely used for studying ubiquitination mechanisms, analyzing tumor signaling pathways, and exploring skin disease models.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RNF187 antibody 32939-1-AP | Download protocol |
| IP protocol for RNF187 antibody 32939-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |