Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue, human testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
32956-1-AP targets RNF185 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38937 Product name: Recombinant human RNF185 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-130 aa of NM_152267.3 Sequence: MASKGPSASASPENSSAGGPSGSSNGAGESGGQDSTFECNICLDTAKDAVISLCGHLFCWPCLHQWLETRPNRQVCPVCKAGISRDKVIPLYGRGSTGQQDPREKTPPRPQGQRPEPENRGGFQGFGFGD 相同性解析による交差性が予測される生物種 |
| Full Name | ring finger protein 185 |
| Calculated molecular weight | 20kDa，192aa |
| Observed molecular weight | 22 kDa |
| GenBank accession number | NM_152267.3 |
| Gene Symbol | RNF185 |
| Gene ID (NCBI) | 91445 |
| RRID | AB_3742804 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q96GF1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RNF185 is a RING finger domain-containing ubiquitin ligase implicated in ER-associated degradation. It is the MOM E3 ligase responsible for regulation of mitochondrial autophagy (PMID: 21931693).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF185 antibody 32956-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |