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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNF180 Polyclonal antibody (30941-1-AP)

    WB analysis using 30941-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30941-1-AP (RNF180 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using RNF180 Polyclonal antibody (30941-1-AP)

    IHC staining of mouse kidney using 30941-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30941-1-AP (RNF180 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RNF180 Polyclonal antibody (30941-1-AP)

    IF Staining of A431 using 30941-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RNF180 antibody (30941-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

RNF180 Polyclonal antibody

RNF180 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30941-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

RNF 180, RING finger protein 180, RINES, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF180

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue, mouse kindey tissue, mouse liver tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30941-1-AP targets RNF180 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF180 fusion protein Ag34138 相同性解析による交差性が予測される生物種
Full Name ring finger protein 180
Calculated molecular weight 592 aa, 68 kDa
Observed molecular weight70 kDa,48kDa
GenBank accession numberBC101277
Gene Symbol RNF180
Gene ID (NCBI) 285671
RRIDAB_3669788
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ86T96
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RNF180 antibody 30941-1-APDownload protocol
IHC protocol for RNF180 antibody 30941-1-APDownload protocol
IF protocol for RNF180 antibody 30941-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols