Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells, human brain tissue, Jurkat cells, Transfected HEK-293 cells |
| Positive IP detected in | HepG2 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 13 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
| IP | See 4 publications below |
| CoIP | See 2 publications below |
Product Information
21393-1-AP targets RNF168 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16055 Product name: Recombinant human RNF168 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 221-571 aa of BC033791 Sequence: NTGDIQKYLTPKSQFGSASHSEAVQEVRKDSVSKDIDSSDRKSPTGQDTEIEDMPTLSPQISLGVGEQGADSSIESPMPWLCACGAEWYHEGNVKTRPSNHGKELCVLSHERPKTRVPYSKETAVMPCGRTESGCAPTSGVTQTNGNNTGETENEESCLLISKEISKRKNQESSFEAVKDPCFSAKRRKVSPESSPDQEETEINFTQKLIDLEHLLFERHKQEEQDRLLALQLQKEVDKEQMVPNRQKGSPDEYHLRATSSPPDKVLNGQRKNPKDGNFKRQTHTKHPTPERGSRDKNRQVSLKMQLKQSVNRRKMPNSTRDHCKVSKSAHSLQPSISQKSVFQMFQRCTK 相同性解析による交差性が予測される生物種 |
| Full Name | ring finger protein 168 |
| Calculated molecular weight | 571 aa, 65 kDa |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | BC033791 |
| Gene Symbol | RNF168 |
| Gene ID (NCBI) | 165918 |
| RRID | AB_10733883 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IYW5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RNF168 antibody 21393-1-AP | Download protocol |
| IP protocol for RNF168 antibody 21393-1-AP | Download protocol |
| WB protocol for RNF168 antibody 21393-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Res SENP1 decreases RNF168 phase separation to promote DNA damage repair and drug resistance in colon cancer | ||
Nat Commun Non-canonical function of DGCR8 in DNA double-strand break repair signaling and tumor radioresistance. | ||
Mol Cell A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis | ||
Nucleic Acids Res The HDAC6-RNF168 axis regulates H2A/H2A.X ubiquitination to enable double-strand break repair
| ||
Nucleic Acids Res GLP-catalyzed H4K16me1 promotes 53BP1 recruitment to permit DNA damage repair and cell survival. | ||
Theranostics A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis. |