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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RNF144B/IBRDC2 Polyclonal antibody (26306-1-AP)

    WB analysis of HeLa using 26306-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26306-1-AP (RNF144B/IBRDC2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using RNF144B/IBRDC2 Polyclonal antibody (26306-1-AP)

    IP experiment of HeLa using 26306-1-AP

    IP result of anti-RNF144B/IBRDC2 (IP:26306-1-AP, 4ug; Detection:26306-1-AP 1:300) with HeLa cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using RNF144B/IBRDC2 Polyclonal antibody (26306-1-AP)

    IHC staining of human kidney using 26306-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26306-1-AP (RNF144B/IBRDC2 antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using RNF144B/IBRDC2 Polyclonal antibody (26306-1-AP)

    IHC staining of human kidney using 26306-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26306-1-AP (RNF144B/IBRDC2 antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using RNF144B/IBRDC2 Polyclonal antibody (26306-1-AP)

    IHC staining of mouse kidney using 26306-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26306-1-AP (RNF144B/IBRDC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using RNF144B/IBRDC2 Polyclonal antibody (26306-1-AP)

    IHC staining of human placenta using 26306-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26306-1-AP (RNF144B/IBRDC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse adrenal gland tissue using RNF144B/IBRDC2 Polyclonal antibody (26306-1-AP)

    IHC staining of mouse adrenal gland using 26306-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 26306-1-AP (RNF144B/IBRDC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat adrenal gland tissue using RNF144B/IBRDC2 Polyclonal antibody (26306-1-AP)

    IHC staining of rat adrenal gland using 26306-1-AP

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 26306-1-AP (RNF144B/IBRDC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RNF144B/IBRDC2 Polyclonal antibody

RNF144B/IBRDC2 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 26306-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, CoIP, ELISA

Synonyms

IBRDC2, RNF144B, E3 ubiquitin-protein ligase RNF144B, EC:2.3.2.31, IBR domain-containing protein 2

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, mouse kidney tissue, rat adrenal gland tissue, mouse adrenal gland tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26306-1-AP targets RNF144B/IBRDC2 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag23679

Product name: Recombinant human RNF144B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 123-187 aa of BC063311

Sequence: VADCQTVCPVASSDPGQPVLVECPSCHLKFCSCCKDAWHAEVSCRDSQPIVLPTEHRALFGTDAE

相同性解析による交差性が予測される生物種
Full Name ring finger protein 144B
Observed molecular weight 34 kDa
GenBank accession numberBC063311
Gene Symbol RNF144B
Gene ID (NCBI) 255488
RRIDAB_2880473
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z419
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for RNF144B/IBRDC2 antibody 26306-1-APDownload protocol
IP protocol for RNF144B/IBRDC2 antibody 26306-1-APDownload protocol
WB protocol for RNF144B/IBRDC2 antibody 26306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP,CoIP

EMBO Rep

RNF144B negatively regulates antiviral immunity by targeting MDA5 for autophagic degradation

Authors - Guoxiu Li
View Article
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