Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse kidney tissue, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
25165-1-AP targets RNF112 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RNF112 fusion protein Ag18306 相同性解析による交差性が予測される生物種 |
| Full Name | ring finger protein 112 |
| Calculated molecular weight | 631 aa, 68 kDa |
| Observed molecular weight | 68 kDa |
| GenBank accession number | BC053989 |
| Gene Symbol | RNF112 |
| Gene ID (NCBI) | 7732 |
| RRID | AB_2879935 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9ULX5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF112 antibody 25165-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
JCI Insight RNF112-mediated FOXM1 ubiquitination suppresses the proliferation and invasion of gastric cancer |