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Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of BxPC-3 cells using RNASET2 Polyclonal antibody (13753-1-AP)

    IP experiment of BxPC-3 using 13753-1-AP (same clone as 13753-1-PBS)

    IP result of anti-RNase T2 (IP:13753-1-AP, 4ug; Detection:13753-1-AP 1:300) with BxPC-3 cells lysate 4000ug. This data was developed using the same antibody clone with 13753-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using RNASET2 Polyclonal antibody (13753-1-AP)

    IHC staining of mouse spleen using 13753-1-AP (same clone as 13753-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 13753-1-AP (RNASET2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13753-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using RNASET2 Polyclonal antibody (13753-1-AP)

    IHC staining of rat small intestine using 13753-1-AP (same clone as 13753-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 13753-1-AP (RNASET2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13753-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of transfected cells using RNASET2 Polyclonal antibody (13753-1-AP)

    IF Staining of transfected cells using 13753-1-AP (same clone as 13753-1-PBS)

    IF result of 13753-1-AP (anti-RNASET2) in COS7 cell by Dr. Nancy Kedersha. COS7 cells transiently transfected with FAST-KD4-YFP, counterstained with PTG RNASET2 antibody (red) and human anti-mitochondrial antigen in blue. Note that FAST-KD4-YFP overexpression alters mitchonrial morphology, and is also present at stress granules (green only). This data was developed using the same antibody clone with 13753-1-PBS in a different storage buffer formulation.

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RNASET2 Polyclonal antibody, PBS Only

RNASET2 Polyclonal Antibody for IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13753-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, IP, Indirect ELISA

Synonyms

RNase T2, RNASE6PL, Ribonuclease T2, Ribonuclease 6, EC:4.6.1.19

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13753-1-PBS targets RNASET2 in IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNASET2 fusion protein Ag4695 相同性解析による交差性が予測される生物種
Full Name ribonuclease T2
Calculated molecular weight 256 aa, 29 kDa
Observed molecular weight 29 kDa
GenBank accession numberBC039713
Gene Symbol RNase T2
Gene ID (NCBI) 8635
RRIDAB_2285339
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00584
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RNASET2(Ribonuclease T2) is also named as RNASE6PL(Ribonuclease 6) and belongs to the RNase T2 family. It is a unique member of the growing family of tumor-antagonizing genes and behaves as a class II tumor suppressor and abolish the tumorigenic potential of an ovarian cancer-derived cell line. This protein has 2 isoforms produced by alternative splicing. Both mildly glycosylated (ranging from38 to 45 kDa) and highly glycosylated forms (ranging from 50 to 80 kDa) of human tumor suppressor protein RNASET2 can be detected in immunoblotting in P. Pastoris(PMID: 21446958).