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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RNASE4 Polyclonal antibody

RNASE4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30732-1-AP

Synonyms

Ribonuclease 4, RNase 4, RNASE4, RNS4

Validation Data Gallery

Applications:
  • WB analysis using 30732-1-AP

    WB analysis using 30732-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30732-1-AP (RNASE4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 30732-1-AP

    WB analysis using 30732-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30732-1-AP (RNASE4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of rat lung using 30732-1-AP

    IHC staining of rat lung using 30732-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 30732-1-AP (RNASE4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat lung using 30732-1-AP

    IHC staining of rat lung using 30732-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 30732-1-AP (RNASE4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse bladder using 30732-1-AP

    IHC staining of mouse bladder using 30732-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 30732-1-AP (RNASE4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman milk, THP-1 cells
Positive IHC detected inrat lung tissue, mouse bladder tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

30732-1-AP targets RNASE4 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNASE4 fusion protein Ag29272 相同性解析による交差性が予測される生物種
Full Name ribonuclease, RNase A family, 4
Observed molecular weight17-20 kDa
GenBank accession numberBC015520
Gene symbol RNASE4
Gene ID (NCBI) 6038
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human Ribonuclease 4 (RNase 4), one of the eight members of the human RNase A superfamily of endoribonucleases, is a uridine-specific endoribonuclease with a preference to cleave RNA after uridine residues prior to purines (PMID: 33818125). RNase 4 is a newly identified host defense peptide in the human kidney and bladder and kills uropathogenic E. coli (UPEC) and multi-drug resistant UPEC (PMID: 28955965).

Protocols

Product Specific Protocols
WB protocol for RNASE4 antibody 30732-1-APDownload protocol
IHC protocol for RNASE4 antibody 30732-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols