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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RNASE2/EDN Polyclonal antibody (18172-1-AP)

    WB analysis using 18172-1-AP (same clone as 18172-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18172-1-AP (RNASE2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18172-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using RNASE2/EDN Polyclonal antibody (18172-1-AP)

    IHC staining of human colon using 18172-1-AP (same clone as 18172-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 18172-1-AP (RNASE2 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18172-1-PBS in a different storage buffer formulation.

RNASE2/EDN Polyclonal antibody, PBS Only

RNASE2/EDN Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 18172-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

RNASE2, RNase UpI-2, RNase 2, Non-secretory ribonuclease, Eosinophil-derived neurotoxin

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

18172-1-PBS targets RNASE2/EDN in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag12834

Product name: Recombinant human RNASE2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 31-161 aa of BC093678

Sequence: QFTWAQWFETQHINMTSQQCTNAMQVINNYQRRCKNQNTFLLTTFANVVNVCGNPNMTCPSNKTRKNCHHSGSQVPLIHCNLTTPSPQNISNCRYAQTPANMFYIVACDNRDQRRDPPQYPVVPVHLDRII

相同性解析による交差性が予測される生物種
Full Name ribonuclease, RNase A family, 2 (liver, eosinophil-derived neurotoxin)
Calculated molecular weight 161 aa, 18 kDa
Observed molecular weight18 kDa
GenBank accession numberBC093678
Gene Symbol RNASE2
Gene ID (NCBI) 6036
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP10153
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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