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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RMND5A Polyclonal antibody (17559-1-AP)

    WB analysis using 17559-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17559-1-AP (RMND5A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RMND5A Polyclonal antibody (17559-1-AP)

    IHC staining of human breast cancer using 17559-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RMND5A Polyclonal antibody (17559-1-AP)

    IHC staining of human breast cancer using 17559-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RMND5A Polyclonal antibody (17559-1-AP)

    IHC staining of human cervical cancer using 17559-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RMND5A Polyclonal antibody (17559-1-AP)

    IHC staining of human cervical cancer using 17559-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RMND5A Polyclonal antibody (17559-1-AP)

    IHC staining of human cervical cancer using 17559-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RMND5A Polyclonal antibody (17559-1-AP)

    IHC staining of human cervical cancer using 17559-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17559-1-AP (RMND5A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • Featured Product
  • KD/KO Validated

RMND5A Polyclonal antibody

RMND5A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17559-1-AP
Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

RMD5, Protein RMD5 homolog A, P44CTLH, EC:2.3.2.27, E3 ubiquitin-protein transferase RMND5A

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17559-1-AP targets RMND5A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RMND5A fusion protein Ag11751 相同性解析による交差性が予測される生物種
Full Name required for meiotic nuclear division 5 homolog A (S. cerevisiae)
Calculated molecular weight 391 aa, 44 kDa
Observed molecular weight40-44 kDa
GenBank accession numberBC047668
Gene Symbol RMND5A
Gene ID (NCBI) 64795
RRIDAB_2878416
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H871
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RMND5A antibody 17559-1-APDownload protocol
IHC protocol for RMND5A antibody 17559-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancers (Basel)

Oncopeptide MBOP Encoded by LINC01234 Promotes Colorectal Cancer through MAPK Signaling Pathway.

Authors - Chunyuan Tang
  • KD Validated
View Article
humanWB

Chembiochem

Identifying E3 ligase substrates with quantitative degradation proteomics

Authors - Victoria N Jordan
View Article
humanWB

Mol Cell

mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway

Authors - Sang Ah Yi
  • KO Validated
View Article