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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RLBP1 Recombinant antibody (85697-1-RR)

    WB analysis using 85697-1-RR (same clone as 85697-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85697-1-RR (RLBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85697-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of ARPE-19 cells using RLBP1 Recombinant antibody (85697-1-RR)

    WB analysis of ARPE-19 using 85697-1-RR (same clone as 85697-1-PBS)

    ARPE-19 cells were subjected to SDS PAGE followed by western blot with 85697-1-RR (RLBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85697-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using RLBP1 Recombinant antibody (85697-1-RR)

    IHC staining of mouse eye using 85697-1-RR (same clone as 85697-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85697-1-RR (RLBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85697-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using RLBP1 Recombinant antibody (85697-1-RR)

    IHC staining of mouse eye using 85697-1-RR (same clone as 85697-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85697-1-RR (RLBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85697-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using RLBP1 Recombinant antibody (85697-1-RR)

    IF Staining of mouse eye using 85697-1-RR (same clone as 85697-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using RLBP1 antibody (85697-1-RR, Clone: 242989D1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85697-1-PBS in a different storage buffer formulation.

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RLBP1 Recombinant antibody, PBS Only

RLBP1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 85697-1-PBS
Clone No.242989D1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Cellular retinaldehyde-binding protein, CRALBP, Retinaldehyde-binding protein 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85697-1-PBS targets RLBP1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RLBP1 fusion protein Ag7602 相同性解析による交差性が予測される生物種
Full Name retinaldehyde binding protein 1
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC004199
Gene Symbol RLBP1
Gene ID (NCBI) 6017
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12271
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RLBP1 (Retinaldehyde-binding protein 1) is also named as cellular retinaldehyde-binding protein (CRALBP). CRALBP, a 36-kDa aqueous soluble carrier, is expressed in abundance by both retinal pigment epithelial (RPE) and Muller cells of retina and is important for rod- and cone-driven vision (PMID: 32188692). Retinal RLBP1 expression was decreased in diabetes, and its overexpression in Müller glia mitigated DR-associated neurovascular degeneration (PMID: 33674409).