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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RIOK3 Recombinant antibody (85449-5-RR)

    WB analysis using 85449-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85449-5-RR (RIOK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RIOK3 Recombinant monoclonal antibody (85449-5-RR)

    IHC staining of human breast cancer using 85449-5-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85449-5-RR (RIOK3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RIOK3 Recombinant monoclonal antibody (85449-5-RR)

    IHC staining of human breast cancer using 85449-5-RR

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 85449-5-RR (RIOK3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human rectal cancer tissue using RIOK3 Recombinant monoclonal antibody (85449-5-RR)

    IHC staining of human rectal cancer using 85449-5-RR

    Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 85449-5-RR (RIOK3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using RIOK3 Recombinant monoclonal antibody (85449-5-RR)

    IF Staining of Jurkat using 85449-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using RIOK3 antibody (85449-5-RR, Clone: 242996A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RIOK3 Recombinant monoclonal antibody (85449-5-RR)

    IF Staining of A431 using 85449-5-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RIOK3 antibody (85449-5-RR, Clone: 242996A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using RIOK3 Recombinant monoclonal antibody (85449-5-RR)

    IF Staining of MCF-7 using 85449-5-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using RIOK3 antibody (85449-5-RR, Clone: 242996A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85449-5-RR

    Affinity and Kinetic Characterization of 85449-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85449-5-RR against Human RIOK3 were performed. The affinity constant is below 1 pM.

RIOK3 Recombinant monoclonal antibody

RIOK3 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85449-5-RR
Clone No.242996A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

242996A6, EC:2.7.11.1, RIO kinase 3, RIO kinase 3 (yeast), Serine/threonine-protein kinase RIO3

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, MCF-7 cells, HeLa cells, HEK-293T cells, HepG2 cells
Positive IHC detected inhuman rectal cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inJurkat cells, A431 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85449-5-RR targets RIOK3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag4497

Product name: Recombinant human RIOK3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 215-516 aa of BC039729

Sequence: RRSARLHEKKEHSTAEKAVDPKTRLLMYKMVNSGMLETITGCISTGKESVVFHAYGGSMEDEKEDSKVIPTECAIKVFKTTLNEFKNRDKYIKDDFRFKDRFSKLNPRKIIRMWAEKEMHNLARMQRAGIPCPTVVLLKKHILVMSFIGHDQVPAPKLKEVKLNSEEMKEAYYQTLHLMRQLYHECTLVHADLSEYNMLWHAGKVWLIDVSQSVEPTHPHGLEFLFRDCRNVSQKGGVKEALSERELFNAVSGLNITADNEADFLAEIEALEKMNEDHVQKNGRKAASFLKDDGDPPLLYDE

相同性解析による交差性が予測される生物種
Full Name RIO kinase 3 (yeast)
Calculated molecular weight 516 aa, 60 kDa
Observed molecular weight60-65 kDa
GenBank accession numberBC039729
Gene Symbol RIOK3
Gene ID (NCBI) 8780
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO14730
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for RIOK3 antibody 85449-5-RRDownload protocol
IHC protocol for RIOK3 antibody 85449-5-RRDownload protocol
WB protocol for RIOK3 antibody 85449-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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