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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RIOK3 Recombinant antibody (85449-5-RR)

    WB analysis using 85449-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85449-5-RR (RIOK3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using RIOK3 Recombinant antibody (85449-5-RR)

    IF Staining of Jurkat using 85449-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using RIOK3 antibody (85449-5-RR, Clone: 242996A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85449-5-RR

    Affinity and Kinetic Characterization of 85449-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85449-5-RR against Human RIOK3 were performed. The affinity constant is below 1 pM.

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RIOK3 Recombinant antibody

RIOK3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85449-5-RR
Clone No.242996A6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

242996A6, EC:2.7.11.1, RIO kinase 3, RIO kinase 3 (yeast), Serine/threonine-protein kinase RIO3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, MCF-7 cells, HeLa cells, HEK-293T cells, HepG2 cells
Positive IF/ICC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85449-5-RR targets RIOK3 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RIOK3 fusion protein Ag4497 相同性解析による交差性が予測される生物種
Full Name RIO kinase 3 (yeast)
Calculated molecular weight 516 aa, 60 kDa
Observed molecular weight60-65 kDa
GenBank accession numberBC039729
Gene Symbol RIOK3
Gene ID (NCBI) 8780
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14730
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RIOK3 antibody 85449-5-RRDownload protocol
IF protocol for RIOK3 antibody 85449-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols