Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, HEK-293 cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85668-5-RR targets RIOK1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag11057 Product name: Recombinant human RIOK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 219-568 aa of BC006104 Sequence: DRDKYVSGEFRFRHGYCKGNPRKMVKTWAEKEMRNLIRLNTAEIPCPEPIMLRSHVLVMSFIGKDDMPAPLLKNVQLSESKARELYLQVIQYMRRMYQDARLVHADLSEFNMLYHGGGVYIIDVSQSVEHDHPHALEFLRKDCANVNDFFMRHSVAVMTVRELFEFVTDPSITHENMDAYLSKAMEIASQRTKEERSSQDHVDEEVFKRAYIPRTLNEVKNYERDMDIIMKLKEEDMAMNAQQDNILYQTVTGLKKDLSGVQKVPALLENQVEERTCSDSEDIGSSECSDTDSEEQGDHARPKKHTTDPDIDKKERKKMVKEAQREKRKNKIPKHVKKRKEKTAKTKKGK 相同性解析による交差性が予測される生物種 |
| Full Name | RIO kinase 1 (yeast) |
| Calculated molecular weight | 568 aa, 66 kDa |
| Observed molecular weight | 75-80 kDa |
| GenBank accession number | BC006104 |
| Gene Symbol | RIOK1 |
| Gene ID (NCBI) | 83732 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9BRS2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RIOK1 (RIO Kinase 1) is a protein-coding gene located on chromosome 6 in humans. It encodes a serine/threonine-protein kinase that belongs to the RIO family of atypical protein kinases/ATPases. This family also includes RIOK2, RIOK3, and RIOB, which have evolved from Archaea to humans. RIOK1 is best known for its role in ribosome biogenesis and its involvement in various cellular processes, including translation regulation and cell-cycle progression. (PMID: 37301535, PMID: 37935656)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RIOK1 antibody 85668-5-RR | Download protocol |
| WB protocol for RIOK1 antibody 85668-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |