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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RIN2 Polyclonal antibody (31223-1-AP)

    WB analysis using 31223-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31223-1-AP (RIN2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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RIN2 Polyclonal antibody

RIN2 Polyclonal Antibody for WB, ELISA
Cat No. 31223-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Ras and Rab interactor 2, Ras inhibitor JC265, RASSF4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, L02 cells, LNCaP cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31223-1-AP targets RIN2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RIN2 fusion protein Ag35011 相同性解析による交差性が予測される生物種
Full Name Ras and Rab interactor 2
Calculated molecular weight100 kDa
Observed molecular weight100-120 kDa
GenBank accession numberNM_018993
Gene Symbol RIN2
Gene ID (NCBI) 54453
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WYP3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RIN2 (Ras and Rab interactor 2), also known as RASSF4. RIN2 is mainly located in Golgi apparatus and participates in the process of material transport and secretion in cells. It is also distributed in cytoplasmic matrix, which may be related to signal transduction in cells. It is highly expressed in the brain, and also in the cerebellum, spinal cord and other nervous system tissues. RIN2 belongs to the family of Ras and Rab exchange factors and has a unique VPS9 domain. It can be used as a nucleotide exchange factor to activate the downstream small GTP enzymes Rab5 and R-Ras, thus starting intracellular signal transduction and regulating cytoskeleton recombination, synapse secretion and cell adhesion. The molecular weight of RIN2 is 100 kDa.

Protocols

Product Specific Protocols
WB protocol for RIN2 antibody 31223-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols