Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse eye tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
Product Information
26111-1-AP targets RIMKLB in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23631 Product name: Recombinant human RIMKLB protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-307 aa of BC033793 Sequence: MCSSVAAKLWFLTDRRIREDYPQKEILRALKAKCCEEELDFRAVVMDEVVLTIEQGNLGLRINGELITAYPQVVVVRVPTPWVQSDSDITVLRHLEKMGCRLMNRPQAILNCVNKFWTFQELAGHGVPLPDTFSYGGHENFAKMIDEAEVLEFPMVVKNTRGHRGKAVFLARDKHHLADLSHLIRHEAPYLFQKYVKESHGRDVRVIVVGGRVVGTMLRCSTDGRMQSNCSLGGVGMMCSLSEQGKQLAIQVSNILGMDVCGIDLLMKDDGSFCVCEANANPFQEPKKQIQTNKKIPREKTFLLPPS 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal modification protein rimK-like family member B |
| Calculated molecular weight | 42 kDa |
| Observed molecular weight | 42-48 kDa |
| GenBank accession number | BC033793 |
| Gene Symbol | RIMKLB |
| Gene ID (NCBI) | 57494 |
| RRID | AB_2880383 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9ULI2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RIMKLB antibody 26111-1-AP | Download protocol |
| IHC protocol for RIMKLB antibody 26111-1-AP | Download protocol |
| IF protocol for RIMKLB antibody 26111-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res Comprehensive metabolomics expands precision medicine for triple-negative breast cancer.
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Sci Bull (Beijing) Tumor cell-derived N-acetyl-aspartyl-glutamate reshapes the tumor microenvironment to facilitate breast cancer metastasis
| ||
J Inherit Metab Dis NAAG synthetase deficiency has only low influence on pathogenesis in a Canavan disease mouse model |