Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells |
| Positive IHC detected in | human ovarian cancer Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82996-4-RR targets RILP in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Affinity | KD=1.04 x 10-11M KOff=2.33 x 10-5M KOn=2.05 x 106M |
| Immunogen |
CatNo: Ag4472 Product name: Recombinant human RILP protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 102-401 aa of BC031621 Sequence: LRAGPQEERALLRQLKEVTDRQRDELRAHNRDLRQRGQETEALQEQLQRLLLVNAELRHKLAAMQTQLRAAQDRERERQQPGEAATPQAKERARGQAGRPGHQHGQEPEWATAGAGAPGNPEDPAEAAQQLGRPSEAGQCRFSREEFEQILQERNELKAKVFLLKEELAYFQRELLTDHRVPGLLLEAMKVAVRKQRKKIKAKMLGTPEEAESSEDEAGPWILLSDDKGDHPPPPESKIQSFFGLWYRGKAESSEDETSSPAPSKLGGEEEAQPQSPAPDPPCSALHEHLCLGASAAPEA 相同性解析による交差性が予測される生物種 |
| Full Name | Rab interacting lysosomal protein |
| Calculated molecular weight | 401 aa, 41 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC031621 |
| Gene Symbol | RILP |
| Gene ID (NCBI) | 83547 |
| RRID | AB_3670743 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96NA2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RILP antibody 82996-4-RR | Download protocol |
| WB protocol for RILP antibody 82996-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
