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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RIF1 Polyclonal antibody (30119-1-AP)

    WB analysis using 30119-1-AP (same clone as 30119-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30119-1-AP (RIF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30119-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RIF1 Polyclonal antibody (30119-1-AP)

    IHC staining of human cervical cancer using 30119-1-AP (same clone as 30119-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 30119-1-AP (RIF1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30119-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RIF1 Polyclonal antibody (30119-1-AP)

    IHC staining of human cervical cancer using 30119-1-AP (same clone as 30119-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 30119-1-AP (RIF1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30119-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RIF1 Polyclonal antibody (30119-1-AP)

    IHC staining of mouse testis using 30119-1-AP (same clone as 30119-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30119-1-AP (RIF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30119-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RIF1 Polyclonal antibody (30119-1-AP)

    IF Staining of HeLa using 30119-1-AP (same clone as 30119-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RIF1 antibody (30119-1-AP) at dilution of 1:1200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30119-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using RIF1 Polyclonal antibody (30119-1-AP)

    IF Staining of HEK-293 using 30119-1-AP (same clone as 30119-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using RIF1 antibody (30119-1-AP) at dilution of 1:1200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 30119-1-PBS in a different storage buffer formulation.

RIF1 Polyclonal antibody, PBS Only

RIF1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 30119-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30119-1-PBS targets RIF1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag32422

Product name: Recombinant human RIF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2277-2472 aa of NM_001177663.1

Sequence: SEMAKESIPCPTESVYPPLVNCVAPVDIILPQITSNMWARGLGQLIRAKNIKTIGDLSTLTASEIKTLPIRSPKVSNVKKALRIYHEQQVKTRGLEEIPVFDISEKTVNGIENKSLSPDEERLVSDIIDPVALEIPLSKNLLAQISALALQLDSEDLHNYSGSQLFEMHEKLSCMANSVIKNLQSRWRSPSHENSI

相同性解析による交差性が予測される生物種
Full Name RAP1 interacting factor homolog (yeast)
Calculated molecular weight274 kDa
Observed molecular weight300 kDa
GenBank accession numberNM_001177663.1
Gene Symbol RIF1
Gene ID (NCBI) 55183
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5UIP0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RIF1 (Rap1-interacting factor 1) is a multifunctional chromosome stability protein implicated in controlling DNA replication and repair. Human RIF1 can interact with protein phosphatase 1 (PP1) and the interaction is crucial for controlling DNA replication by limiting phosphorylation of the MCM complex (PMID: 28077461). RIF1 also acts with PP1 to protect nascent DNA from overdegradation at stalled replication forks (PMID: 31141682). RIF1 promotes non-homologous end joining (NHEJ) in double strand break (DSB) repair (PMID: 30443189). Research studies show that RIF1 is overexpressed in breast cancer tissues and is upgraded in NSCLC tissues (PMID: 30237512).

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