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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ARHGAP32 Polyclonal antibody (15024-1-AP)

    WB analysis using 15024-1-AP

    HeLa cells were subjected to Tris-Acetate gel system followed by western blot with 15024-1-AP (ARHGAP32 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ARHGAP32 Polyclonal antibody (15024-1-AP)

    IHC staining of mouse testis using 15024-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15024-1-AP (ARHGAP32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ARHGAP32 Polyclonal antibody (15024-1-AP)

    IHC staining of mouse testis using 15024-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15024-1-AP (ARHGAP32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ARHGAP32 Polyclonal antibody (15024-1-AP)

    IHC staining of mouse brain using 15024-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15024-1-AP (ARHGAP32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ARHGAP32 Polyclonal antibody (15024-1-AP)

    IHC staining of mouse brain using 15024-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15024-1-AP (ARHGAP32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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ARHGAP32 Polyclonal antibody

ARHGAP32 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15024-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

ARHGAP32, GC GAP, GRIT, KIAA0712, p200RhoGAP, p250GAP, Rho GTPase activating protein, RICS

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inmouse testis tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15024-1-AP targets ARHGAP32 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ARHGAP32 fusion protein Ag7104 相同性解析による交差性が予測される生物種
Full Name Rho GTPase-activating protein
Calculated molecular weight 231 kDa
Observed molecular weight220 kDa
GenBank accession numberBC000277
Gene Symbol ARHGAP32
Gene ID (NCBI) 9743
RRIDAB_3085448
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDA7KAX9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ARHGAP32, also known as RICS, belongs to the PX domain-containing GAP family. ARHGAP32 encodes a neuron-associated GTPase-activating protein that regulates dendritic spine morphology and strength. ARHGAP32 also can interact with RhoA and β-catenin, thereby inhibiting phosphorylation of LIMK/cofilin and promoting epithelial-to-mesenchymal transition (EMT) process. ARHGAP32 is highly expressed in brain and testis (PMID: 30888095, PMID: 32205841).

Protocols

Product Specific Protocols
WB protocol for ARHGAP32 antibody 15024-1-APDownload protocol
IHC protocol for ARHGAP32 antibody 15024-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Nutr Biochem

Hippocampal proteomic changes in high-fat diet-induced obese mice associated with memory decline

Authors - Ping Lu
View Article