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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RIBC2 Polyclonal antibody (15272-1-AP)

    WB analysis using 15272-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15272-1-AP (RIBC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using RIBC2 Polyclonal antibody (15272-1-AP)

    WB analysis of human brain using 15272-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15272-1-AP (RIBC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using RIBC2 Polyclonal antibody (15272-1-AP)

    WB analysis of human brain using 15272-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15272-1-AP (RIBC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using RIBC2 Polyclonal antibody (15272-1-AP)

    IHC staining of human cervical cancer using 15272-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15272-1-AP (RIBC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using RIBC2 Polyclonal antibody (15272-1-AP)

    IHC staining of mouse testis using 15272-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15272-1-AP (RIBC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RIBC2 Polyclonal antibody (15272-1-AP)

    IHC staining of human colon cancer using 15272-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 15272-1-AP (RIBC2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RIBC2 Polyclonal antibody (15272-1-AP)

    IHC staining of human colon cancer using 15272-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 15272-1-AP (RIBC2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RIBC2 Polyclonal antibody (15272-1-AP)

    IF Staining of HeLa using 15272-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using RIBC2 antibody (15272-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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RIBC2 Polyclonal antibody

RIBC2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15272-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C22orf11, RIB43A-like with coiled-coils protein 2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected infetal human brain tissue, human brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse testis tissue, human cervical cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15272-1-AP targets RIBC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RIBC2 fusion protein Ag7385 相同性解析による交差性が予測される生物種
Full Name RIB43A domain with coiled-coils 2
Calculated molecular weight 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC003024
Gene Symbol RIBC2
Gene ID (NCBI) 26150
RRIDAB_2179714
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H4K1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RIBC2 is a microtubule protein and is part of the double microtubules (DMTs) decorated with dynamic proteins in the ciliary axons. It is mainly located in the microtubules of cilia and is involved in flagellated sperm motility(PMID: 40265983).

Protocols

Product Specific Protocols
WB protocol for RIBC2 antibody 15272-1-APDownload protocol
IHC protocol for RIBC2 antibody 15272-1-APDownload protocol
IF protocol for RIBC2 antibody 15272-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols