Validation Data Gallery
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Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
13500-1-AP targets RIAM in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, chicken, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RIAM fusion protein Ag4327 相同性解析による交差性が予測される生物種 |
| Full Name | amyloid beta (A4) precursor protein-binding, family B, member 1 interacting protein |
| Calculated molecular weight | 666 aa, 73 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC035636 |
| Gene Symbol | RIAM |
| Gene ID (NCBI) | 54518 |
| RRID | AB_2057231 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z5R6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
APBB1IP gene encodes a Rap1-GTP-interacting adapter molecule (RIAM) which is an important protein in Rap1-mediated integrin activation and adhesion. Rap1 is a small GTPase frequently activated in tumors such as melanoma and prostate cancer. RIAM is widely expressed with high expression in thymus, spleen, lymph node, bone marrow and peripheral leukocytes. RIAM may function in the signal transduction from Ras activation to actin cytoskeletal remodeling. The observed molecular weight of RIAM is about 100 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RIAM antibody 13500-1-AP | Download protocol |
| IHC protocol for RIAM antibody 13500-1-AP | Download protocol |
| IP protocol for RIAM antibody 13500-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Microbiol Spectr Avian Hepatitis E Virus ORF2 Protein Interacts with Rap1b to Induce Cytoskeleton Rearrangement That Facilitates Virus Internalization.
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PLoS One HPK1 associates with SKAP-HOM to negatively regulate Rap1-mediated B-lymphocyte adhesion. | ||
Immunol Lett Non-cleavable talin rescues defect in the T-cell conjugation of T-cells deficient in the immune adaptor SKAP1. | ||
Oncol Lett Identification of prognostic biomarkers associated with metastasis and immune infiltration in osteosarcoma. |