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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RHOQ/TC10 Monoclonal antibody (67213-1-Ig)

    WB analysis using 67213-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67213-1-Ig (RHOQ/TC10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using RHOQ/TC10 Monoclonal antibody (67213-1-Ig)

    IHC staining of mouse liver using 67213-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67213-1-Ig (RHOQ/TC10 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using RHOQ/TC10 Monoclonal antibody (67213-1-Ig)

    IHC staining of mouse liver using 67213-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 67213-1-Ig (RHOQ/TC10 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RHOQ/TC10 Monoclonal antibody (67213-1-Ig)

    IHC staining of human colon cancer using 67213-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67213-1-Ig (RHOQ/TC10 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RHOQ/TC10 Monoclonal antibody (67213-1-Ig)

    IHC staining of human colon cancer using 67213-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67213-1-Ig (RHOQ/TC10 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RHOQ/TC10 Monoclonal antibody

RHOQ/TC10 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67213-1-Ig
Clone No.3B9C7

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, ELISA

Synonyms

ARHQ, Ras like protein TC10, RASL7A, RHOQ, RHOQ/TC10, TC10, TC10A

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, rat brain tissue, pig brain tissue, fetal human brain tissue, Jurkat cells, A549 cells
Positive IHC detected inmouse liver tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:8000-1:32000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67213-1-Ig targets RHOQ/TC10 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Reactivity Human, mouse, rat, pig
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag12078

Product name: Recombinant human RHOQ protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-205 aa of BC070485

Sequence: MAHGPGALMLKCVVVGDGAVGKTCLLMSYANDAFPEEYVPTVFDHYAVSVTVGGKQYLLGLYDTAGQEDYDRLRPLSYPMTDVFLICFSVVNPASFQNVKEEWVPELKEYAPNVPFLLIGTQIDLRDDPKTLARLNDMKEKPICVEQGQKLAKEIGACCYVECSALTQKGLKTVFDEAIIAILTPKKHTVKKRIGSRCINCCLIT

相同性解析による交差性が予測される生物種
Full Name ras homolog gene family, member Q
Calculated molecular weight 205 aa, 23 kDa
Observed molecular weight 20-23 kDa
GenBank accession numberBC070485
Gene Symbol RHOQ/TC10
Gene ID (NCBI) 23433
RRIDAB_2882505
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP17081
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for RHOQ/TC10 antibody 67213-1-IgDownload protocol
WB protocol for RHOQ/TC10 antibody 67213-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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