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Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using RHOBTB3 Monoclonal antibody (67502-1-Ig)

    WB analysis of K-562 using 67502-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RHOBTB3 Monoclonal antibody (67502-1-Ig)

    WB analysis of Jurkat using 67502-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig brain tissue using RHOBTB3 Monoclonal antibody (67502-1-Ig)

    WB analysis of pig brain using 67502-1-Ig

    pig brain tissue were subjected to SDS PAGE followed by western blot with 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U2OS cells using RHOBTB3 Monoclonal antibody (67502-1-Ig)

    WB analysis of U2OS using 67502-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RHOBTB3 Monoclonal antibody (67502-1-Ig)

    WB analysis of HEK-293 using 67502-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using RHOBTB3 Monoclonal antibody (67502-1-Ig)

    WB analysis of HeLa using 67502-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RHOBTB3 Monoclonal antibody (67502-1-Ig)

    IHC staining of mouse brain using 67502-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat adrenal gland tissue using RHOBTB3 Monoclonal antibody (67502-1-Ig)

    IHC staining of rat adrenal gland using 67502-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RHOBTB3 Monoclonal antibody (67502-1-Ig)

    IHC staining of mouse brain using 67502-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using RHOBTB3 Monoclonal antibody (67502-1-Ig)

    IHC staining of mouse heart using 67502-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using RHOBTB3 Monoclonal antibody (67502-1-Ig)

    IHC staining of mouse heart using 67502-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 67502-1-Ig (RHOBTB3 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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RHOBTB3 Monoclonal antibody

RHOBTB3 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67502-1-Ig
Clone No.1H2E3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Synonyms

Rho-related BTB domain-containing protein 3, KIAA0878, EC:3.6.1.-, 1H2E3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, U2OS cells, HEK-293 cells, HeLa cells, Jurkat cells, pig brain tissue
Positive IHC detected inmouse brain tissue, mouse heart tissue, rat adrenal gland tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67502-1-Ig targets RHOBTB3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RHOBTB3 fusion protein Ag5782 相同性解析による交差性が予測される生物種
Full Name Rho-related BTB domain containing 3
Calculated molecular weight 611 aa, 69 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC041337
Gene Symbol RHOBTB3
Gene ID (NCBI) 22836
RRIDAB_2882726
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO94955
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RHOBTB3 antibody 67502-1-IgDownload protocol
IHC protocol for RHOBTB3 antibody 67502-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols