Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, K-562 cells |
| Positive IHC detected in | mouse heart tissue, human placenta tissue, rat adrenal gland tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IP | See 1 publications below |
Product Information
13945-1-AP targets RHOBTB3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RHOBTB3 fusion protein Ag5025 相同性解析による交差性が予測される生物種 |
| Full Name | Rho-related BTB domain containing 3 |
| Calculated molecular weight | 611 aa, 69 kDa |
| Observed molecular weight | 69 kDa |
| GenBank accession number | BC041337 |
| Gene Symbol | RHOBTB3 |
| Gene ID (NCBI) | 22836 |
| RRID | AB_2877992 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94955 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RHOBTB3 antibody 13945-1-AP | Download protocol |
| IHC protocol for RHOBTB3 antibody 13945-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Theranostics Circular RNA circRHOBTB3 represses metastasis by regulating the HuR-mediated mRNA stability of PTBP1 in colorectal cancer. | ||
Cells RhoBTB3 Functions as a Novel Regulator of Autophagy by Suppressing AMBRA1 Stability |