Validation Data Gallery

Filter: AllWBIHC

WB analysis of HEK-293 using 11949-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11949-1-AP (RHCG antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 11949-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 11949-1-AP (RHCG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas cancer using 11949-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11949-1-AP (RHCG antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 11949-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human kidney using 11949-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human kidney using 11949-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 11949-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 11949-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:25 (under 10x lens).
IHC staining of human kidney using 11949-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 11949-1-AP (RHCG antibody) at dilution of 1:25 (under 40x lens).

Featured Product
KD/KO Validated

RHCG Polyclonal antibody

RHCG Polyclonal Antibody for WB, IHC, ELISA

Cat No. 11949-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Synonyms

Tumor-related protein DRC2, RHGK, PDRC2, CDRC2, C15orf6

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, human kidney tissue
Positive IHC detected in	human kidney tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IHC	See 1 publications below
IF	See 1 publications below
CoIP	See 1 publications below

Product Information

11949-1-AP targets RHCG in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2493 Product name: Recombinant human RHCG protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 240-479 aa of BC030965 Sequence: SYHGDSQHRAAINTYCSLAACVLTSVAISSALHKKGKLDMVHIQNATLAGGVAVGTAAEMMLMPYGALIIGFVCGIISTLGFVYLTPFLESRLHIQDTCGINNLHGIPGIIGGIVGAVTAASASLEVYGKEGLVHSFDFQGFNGDWTARTQGKFQIYGLLVTLAMALMGGIIVGLILRLPFWGQPSDENCFEDAVYWEMPEGNSTVYIPEDPTFKPSGPSVPSVPMVSPLPMASSVPLVP 相同性解析による交差性が予測される生物種
Full Name	Rh family, C glycoprotein
Calculated molecular weight	479 aa, 53 kDa
Observed molecular weight	52-55 kDa
GenBank accession number	BC030965
Gene Symbol	RHCG
Gene ID (NCBI)	51458
RRID	AB_2269482
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UBD6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for RHCG antibody 11949-1-AP	Download protocol
WB protocol for RHCG antibody 11949-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IF,CoIP	Kidney Int The ammonia transporter RhCG modulates urinary acidification by interacting with the vacuolar proton-ATPases in renal intercalated cells. Authors - Soline Bourgeois KO Validated View Article
human	WB,IHC	Aging (Albany NY) Rh type C-glycoprotein functions as a novel tumor suppressor gene by inhibiting tumorigenicity and metastasis in head and neck squamous cell carcinoma. Authors - Wenguang Xu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HEK-293 using 11949-1-AP

WB analysis of human kidney using 11949-1-AP

IHC staining of human pancreas cancer using 11949-1-AP

IHC staining of human kidney using 11949-1-AP

IHC staining of human kidney using 11949-1-AP

IHC staining of human kidney using 11949-1-AP

IHC staining of human kidney using 11949-1-AP

IHC staining of human kidney using 11949-1-AP

IHC staining of human kidney using 11949-1-AP

RHCG Polyclonal antibody

RHCG Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

The ammonia transporter RhCG modulates urinary acidification by interacting with the vacuolar proton-ATPases in renal intercalated cells.

Rh type C-glycoprotein functions as a novel tumor suppressor gene by inhibiting tumorigenicity and metastasis in head and neck squamous cell carcinoma.