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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RHAMM,Lgals9 Polyclonal antibody (15819-1-AP)

    WB analysis using 15819-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15819-1-AP (RHAMM,Lgals9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RHAMM,Lgals9 Polyclonal antibody (15819-1-AP)

    WB analysis of NIH/3T3 using 15819-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15819-1-AP (RHAMM,Lgals9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RHAMM,Lgals9 Polyclonal antibody (15819-1-AP)

    WB analysis of NIH/3T3 using 15819-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15819-1-AP (RHAMM,Lgals9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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RHAMM,Lgals9 Polyclonal antibody

RHAMM,Lgals9 Polyclonal Antibody for WB, ELISA
Cat No. 15819-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

Hmmr, RHAMM

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse spleen tissue, NIH/3T3 cells, rat spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15819-1-AP targets RHAMM,Lgals9 in WB, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name hyaluronan mediated motility receptor (RHAMM)
Calculated molecular weight 84 kDa
Observed molecular weight 32 kDa, 70 kDa
GenBank accession numberNM_012964
Gene Symbol RHAMM
Gene ID (NCBI) 25460
RRIDAB_2137234
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9WUF7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HMMR, also named as RHAMM, is a hyaluronan receptor expressed in various tissues. It is involved in regulation of focal contacts and subsequent motility, thus to play function in wound healing and tumor metastasis. The protein was expressed as a 70kd one in many tissues.

Protocols

Product Specific Protocols
WB protocol for RHAMM,Lgals9 antibody 15819-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols