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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using RHAG Monoclonal antibody (67714-1-Ig)

    WB analysis of human placenta using 67714-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67714-1-Ig (RHAG antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using RHAG Monoclonal antibody (67714-1-Ig)

    IHC staining of human placenta using 67714-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67714-1-Ig (RHAG antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Immunohistochemistry (IHC) staining of human placenta tissue using RHAG Monoclonal antibody (67714-1-Ig)

    IHC staining of human placenta using 67714-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67714-1-Ig (RHAG antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Immunohistochemistry (IHC) staining of human spleen tissue using RHAG Monoclonal antibody (67714-1-Ig)

    IHC staining of human spleen using 67714-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67714-1-Ig (RHAG antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using RHAG Monoclonal antibody (67714-1-Ig)

    IHC staining of human spleen using 67714-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67714-1-Ig (RHAG antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using RHAG Monoclonal antibody (67714-1-Ig)

    IF Staining of human placenta using 67714-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using RHAG antibody (67714-1-Ig, Clone: 1C1C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using RHAG Monoclonal antibody (67714-1-Ig)

    IF Staining of human placenta using 67714-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using RHAG antibody (67714-1-Ig, Clone: 1C1C4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

RHAG Monoclonal antibody

RHAG Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67714-1-Ig
Clone No.1C1C4

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF-P, ELISA

Synonyms

Ammonium transporter Rh type A, CD241, Rh associated glycoprotein, Rh family type A glycoprotein, Rh type A glycoprotein, RH2, Rh50, RH50A, Rh50GP, RHAG, SLC42A1

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67714-1-Ig targets RHAG in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag30130

Product name: Recombinant human RHAG protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-53 aa of BC012605

Sequence: MRFTFPLMAIVLEIAMIVLFGLFVEYETDQTVLEQLNITKPTDMGIFFELYPL

相同性解析による交差性が予測される生物種
Full Name Rh-associated glycoprotein
Calculated molecular weight 409 aa, 44 kDa
Observed molecular weight50-60 kDa
GenBank accession numberBC012605
Gene Symbol RHAG
Gene ID (NCBI) 6005
RRIDAB_2882904
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ02094
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for RHAG antibody 67714-1-IgDownload protocol
IHC protocol for RHAG antibody 67714-1-IgDownload protocol
WB protocol for RHAG antibody 67714-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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