Validation Data Gallery
Tested Applications
| Positive WB detected in | SK-N-SH cells, SH-SY5Y cells, HUVEC cells, NIH/3T3 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
88404-1-RR targets RGS5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag2196 Product name: Recombinant human RGS5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-181 aa of BC030059 Sequence: MCKGLAALPHSCLERAKEIKIKLGILLQKPDSVGDLVIPYNEKPEKPAKTQKTSLDEALQWRDSLDKLLQNNYGLASFKSFLKSEFSEENLEFWIACEDYKKIKSPAKMAEKAKQIYEEFIQTEAPKEVNIDHFTKDITMKNLVEPSLSSFDMAQKRIHALMEKDSLPRFVRSEFYQELIK 相同性解析による交差性が予測される生物種 |
| Full Name | regulator of G-protein signaling 5 |
| Calculated molecular weight | 181 aa, 21 kDa |
| Observed molecular weight | 42 kDa |
| GenBank accession number | BC030059 |
| Gene Symbol | RGS5 |
| Gene ID (NCBI) | 8490 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O15539 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RGS5 inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. It binds to G(i)-alpha and G(o)-alpha, but not to G(s)-alpha. The 42kDa band recognized by this antibody is a RGS5-RGS5 dimer. (PMID:17762159)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RGS5 antibody 88404-1-RR | Download protocol |
| WB protocol for RGS5 antibody 88404-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |