Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
Product Information
66790-1-Ig targets RGS3 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3177 Product name: Recombinant human RGS3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-241 aa of BC018072 Sequence: VIRPGFQRACVAAACTVAARCPGRGVGDRSQSGASYRPICGPKVGGPTEMLRGMYLTRNGNLQRRHTMKEAKDMKNKLGIFRRRSESPGAPPAGKADKMMKSFKPTSEEALKWGESLEKLLVHKYGLAVFQAFLRTEFSEENLEFWLACEDFKKVKSQSKMASKAKKIFAEYIAIQACKEVNLDSYTREHTKDNLQSVTRGCFDLAQKRIFGLMEKDSYPRFLRSDLYLDLINQKKMSPPL 相同性解析による交差性が予測される生物種 |
| Full Name | regulator of G-protein signaling 3 |
| Calculated molecular weight | 1198 aa, 132 kDa |
| Observed molecular weight | 140 kDa |
| GenBank accession number | BC018072 |
| Gene Symbol | RGS3 |
| Gene ID (NCBI) | 5998 |
| RRID | AB_2882134 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P49796 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RGS3 belongs to a family of proteins which share the core regulator of G-protein signalling (RGS) domain and function as GTPase-activating proteins (GAPs) for the α subunit of heterotrimeric G-proteins. RGS3 has been reported to inhibit the Gai-and Gaq-mediated signaling pathways in the cardiovascular system, including those acting via the cardiovascular signaling molecules angiotensin II (Ang II), sphingosine 1-phosphate and endothelin-1. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. Long isoforms are largely cytosolic and plasma membrane-associated with a function in Wnt signaling and in the epithelial mesenchymal transition, while shorter N-terminally-truncated isoforms can be nuclear.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RGS3 antibody 66790-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Transl Cancer Res Proteome-wide identification and functional analysis of ubiquitinated proteins in Hepa1-6 cells by knockdown of E3 ubiquitin ligase SIAH1 | ||
Stem Cell Res Ther Exosomal mir-126-3p derived from endothelial cells induces ion channel dysfunction by targeting RGS3 signaling in cardiomyocytes: a novel mechanism in Takotsubo cardiomyopathy |