Validation Data Gallery
Tested Applications
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28231-1-AP targets RGS22 in IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28156 Product name: Recombinant human RGS22 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 250-499 aa of BC092411 Sequence: HPRTKKDPSKTNKLISEFEEEEGEEEEVSVSLQDTPSQALLRVYLEKKQDVDESLTMHFSTCEEFLSSYIYFILRGAIQQIVGKPVGETPDYINFNNITKVSFDDCFESIHGKNFLSELVQTTKERSEEIEQTSLSSKNESAGPESRADWCISHRTYDIGNRKEFERFKKFIKGTLGERYWWLWMDIERLKVLKDPGRHQRHLEKMKKCYLVSNGDYYLSAEILSKFKLLDGSQWNEEHLRNIQSEVLKP 相同性解析による交差性が予測される生物種 |
| Full Name | regulator of G-protein signaling 22 |
| Calculated molecular weight | 1263 aa, 147 kDa |
| GenBank accession number | BC092411 |
| Gene Symbol | RGS22 |
| Gene ID (NCBI) | 26166 |
| RRID | AB_3742287 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NE09 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The RGS22 protein (Regulator of G-protein signaling 22) belongs to the regulators of G-protein signaling (RGS) protein family and is a member of the RA/RGS subfamily. Its protein structure contains at least three conserved RGS domains. RGS22 primarily functions as a GTPase-activating protein (GAP) by binding to the G protein α subunit and enhancing its GTPase activity, thereby promoting the conversion of G proteins from an active state to an inactive state, which negatively regulates downstream signal transduction of G protein-coupled receptors.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RGS22 antibody 28231-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |