Validation Data Gallery

Filter: AllWBIHC

WB analysis of fetal human brain using 10678-1-AP
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 10678-1-AP (RGS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10678-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10678-1-AP (RGS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas cancer using 10678-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10678-1-AP (RGS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse bladder using 10678-1-AP
Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 10678-1-AP (RGS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse bladder using 10678-1-AP
Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 10678-1-AP (RGS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse pancreas using 10678-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10678-1-AP (RGS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat bladder using 10678-1-AP
Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 10678-1-AP (RGS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat pancreas using 10678-1-AP
Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 10678-1-AP (RGS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat adrenal gland using 10678-1-AP
Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 10678-1-AP (RGS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

RGS2 Polyclonal antibody

RGS2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10678-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

G0S8, Cell growth inhibiting gene 31 protein, Cell growth-inhibiting gene 31 protein, G0/G1 switch regulatory protein 8, GIG31

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (11)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	fetal human brain tissue, human brain tissue
Positive IHC detected in	human pancreas cancer tissue, mouse bladder tissue, mouse pancreas tissue, rat adrenal gland tissue, rat bladder tissue, rat pancreas tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 9 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

10678-1-AP targets RGS2 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1066 Product name: Recombinant human RGS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-211 aa of BC007049 Sequence: MQSAMFLAVQHDCRPMDKSAGSGHKSEEKREKMKRTLLKDWKTRLSYFLQNSSTPGKPKTGKKSKQQAFIKPSPEEAQLWSEAFDELLASKYGLAAFRAFLKSEFCEENIEFWLACEDFKKTKSPQKLSSKARKIYTDFIEKEAPKEINIDFQTKTLIAQNIQEATSGCFTTAQKRVYSLMENNSYPRFLESEFYQDLCKKPQITTEPHAT 相同性解析による交差性が予測される生物種
Full Name	regulator of G-protein signaling 2, 24kDa
Calculated molecular weight	24 kDa
Observed molecular weight	24-30 kDa
GenBank accession number	BC007049
Gene Symbol	RGS2
Gene ID (NCBI)	5997
RRID	AB_2284998
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P41220
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for RGS2 antibody 10678-1-AP	Download protocol
WB protocol for RGS2 antibody 10678-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Clin Invest RGS2-mediated translational control mediates cancer cell dormancy and tumor relapse. Authors - Jaebeom Cho KD Validated View Article
human	WB	Basic Res Cardiol Novel insights into the mechanisms mediating the local antihypertrophic effects of cardiac atrial natriuretic peptide: role of cGMP-dependent protein kinase and RGS2. Authors - Klaiber Michael M View Article
human	WB,IF	J Cell Physiol Novel role of Giα2 in cell migration: Downstream of PI3-kinase-AKT and Rac1 in prostate cancer cells. Authors - Silvia Caggia KD Validated View Article
human	WB	Respir Res Upregulation of RGS2: a new mechanism for pirfenidone amelioration of pulmonary fibrosis. Authors - Yan Xie View Article
rat	WB,IP	Ecotoxicol Environ Saf Protective role of RGS2 in PM2.5-induced ovarian injury in rats: Modulation of Gq/11 signaling to maintain granulosa cell [Ca2 + ]i stability Authors - Zhenhua Ma View Article
human	WB	Hum Mutat Secretory Lysosome-Related Gene Signature Defines the Immune Microenvironment and Identifies RGS2 as a Prometastatic Factor in Hepatocellular Carcinoma. Authors - Zhipeng Ye KD Validated View Article

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

ゲノミクス試薬

研究分野

Validation Data Gallery

WB analysis of fetal human brain using 10678-1-AP

WB analysis of human brain using 10678-1-AP

IHC staining of human pancreas cancer using 10678-1-AP

IHC staining of mouse bladder using 10678-1-AP

IHC staining of mouse bladder using 10678-1-AP

IHC staining of mouse pancreas using 10678-1-AP

IHC staining of rat bladder using 10678-1-AP

IHC staining of rat pancreas using 10678-1-AP

IHC staining of rat adrenal gland using 10678-1-AP

RGS2 Polyclonal antibody

RGS2 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

RGS2-mediated translational control mediates cancer cell dormancy and tumor relapse.

Novel insights into the mechanisms mediating the local antihypertrophic effects of cardiac atrial natriuretic peptide: role of cGMP-dependent protein kinase and RGS2.

Novel role of Giα2 in cell migration: Downstream of PI3-kinase-AKT and Rac1 in prostate cancer cells.

Upregulation of RGS2: a new mechanism for pirfenidone amelioration of pulmonary fibrosis.

Protective role of RGS2 in PM2.5-induced ovarian injury in rats: Modulation of Gq/11 signaling to maintain granulosa cell [Ca2 + ]i stability

Secretory Lysosome-Related Gene Signature Defines the Immune Microenvironment and Identifies RGS2 as a Prometastatic Factor in Hepatocellular Carcinoma.

Able^™

Validation Data Gallery

WB analysis of fetal human brain using 10678-1-AP

WB analysis of human brain using 10678-1-AP

IHC staining of human pancreas cancer using 10678-1-AP

IHC staining of mouse bladder using 10678-1-AP

IHC staining of mouse bladder using 10678-1-AP

IHC staining of mouse pancreas using 10678-1-AP

IHC staining of rat bladder using 10678-1-AP

IHC staining of rat pancreas using 10678-1-AP

IHC staining of rat adrenal gland using 10678-1-AP

RGS2 Polyclonal antibody

RGS2 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

RGS2-mediated translational control mediates cancer cell dormancy and tumor relapse.

Novel insights into the mechanisms mediating the local antihypertrophic effects of cardiac atrial natriuretic peptide: role of cGMP-dependent protein kinase and RGS2.

Novel role of Giα2 in cell migration: Downstream of PI3-kinase-AKT and Rac1 in prostate cancer cells.

Upregulation of RGS2: a new mechanism for pirfenidone amelioration of pulmonary fibrosis.

Protective role of RGS2 in PM2.5-induced ovarian injury in rats: Modulation of Gq/11 signaling to maintain granulosa cell [Ca2 + ]i stability

Secretory Lysosome-Related Gene Signature Defines the Immune Microenvironment and Identifies RGS2 as a Prometastatic Factor in Hepatocellular Carcinoma.

Able™

Protective role of RGS2 in PM2.5-induced ovarian injury in rats: Modulation of Gq/11 signaling to maintain granulosa cell [Ca2 + ]i stability

Able^™