Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | mouse thymus tissue, K-562 cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
Product Information
11163-1-AP targets RGS10 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1644 Product name: Recombinant human RGS10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-181 aa of BC009361 Sequence: MFNRAVSRLSRKRPPSDIHDSDGSSSSSHQSLKSTAKWAASLENLLEDPEGVKRFREFLKKEFSEENVLFWLACEDFKKMQDKTQMQEKAKEIYMTFLSSKASSQVNVEGQSRLNEKILEEPHPLMFQKLQDQIFNLMKYDSYSRFLKSDLFLKHKRTEEEEEDLPDAQTAAKRASRIYNT 相同性解析による交差性が予測される生物種 |
| Full Name | regulator of G-protein signaling 10 |
| Calculated molecular weight | 21 kDa |
| Observed molecular weight | 20-22 kDa |
| GenBank accession number | BC009361 |
| Gene Symbol | RGS10 |
| Gene ID (NCBI) | 6001 |
| RRID | AB_10596933 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43665 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RGS10 antibody 11163-1-AP | Download protocol |
| WB protocol for RGS10 antibody 11163-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int Endod J RGS10 negatively regulates apical periodontitis via TFEB-mediated autophagy in BABL/c mice model and in vitro | ||
Oral Dis Inhibition of Rgs10 aggravates periodontitis with collagen-induced arthritis via the NF-κB pathway.
| ||
J Endod Inhibition of RGS10 Aggravates Periapical Periodontitis via Upregulation of the NF-κB Pathway |
