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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RGS1 Polyclonal antibody (12547-1-AP)

    WB analysis using 12547-1-AP (same clone as 12547-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12547-1-AP (RGS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12547-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using RGS1 Polyclonal antibody (12547-1-AP)

    WB analysis using 12547-1-AP (same clone as 12547-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12547-1-AP (RGS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12547-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using RGS1 Polyclonal antibody (12547-1-AP)

    WB analysis of HepG2 using 12547-1-AP (same clone as 12547-1-PBS)

    WB result of RGS1 antibody (12547-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-RGS1 transfected HepG2 cells. This data was developed using the same antibody clone with 12547-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RGS1 Polyclonal antibody (12547-1-AP)

    IHC staining of human stomach cancer using 12547-1-AP (same clone as 12547-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12547-1-AP (RGS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 12547-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RGS1 Polyclonal antibody, PBS Only

RGS1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12547-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

IER1, Early response protein 1R20, BL34, B-cell activation protein BL34, B cell activation protein BL34

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12547-1-PBS targets RGS1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3315

Product name: Recombinant human RGS1 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-196 aa of BC015510

Sequence: MPGMFFSANPKELKGTTHSLLDDKMQKRRPKTFGMDMKAYLRSMIPHLESGMKSSKSKDVLSAAEVMQWSQSLEKLLANQTGQNVFGSFLKSEFSEENIEFWLACEDYKKTESDLLPCKAEEIYKAFVHSDAAKQINIDFRTRESTAKKIKAPTPTCFDEAQKVIYTLMEKDSYPRFLKSDIYLNLLNDLQANSLK

相同性解析による交差性が予測される生物種
Full Name regulator of G-protein signaling 1
Calculated molecular weight 196 aa, 22 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC015510
Gene Symbol RGS1
Gene ID (NCBI) 5996
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ08116
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

RGS1 (Regulator of G-protein signaling 1) is also named as 1R20, BL34 and IER1. RGS1 encodes a member of the regulator of G-protein signaling family, which can act as a GTPase-activating protein (GAP), increasing the rate of conversion of the GTP to GDP, driving G-protein into its inactive GDP-bound form, hence attenuating or turning off G-protein-coupled receptor signaling (PMID: 8774882). RGS1 is highly expressed in immune cells including T cells, B cells, natural killer (NK) cells, dendritic cells and monocytes, suggesting a role for RGS1 in immune cell regulation (PMID: 34956194). RGS1 was highly expressed in tumor tissues and correlated with shorter overall survival, which also appeared in several previous studies, including multiple myeloma, melanoma, nonsmall cell lung cancer, gastric cancer , diffuse large B-cell lymphoma (PMID: 34956194). RGS1 protein, located at the cytoplasm and membrane, is enriched in tumor tissues compared with normal tissues. RGS1 was identified as a HIF-dependent hypoxia target that dampens cell migration and signal transduction (PMID: 24686421).

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