Validation Data Gallery
Tested Applications
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31636-1-AP targets RGPD5 in IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36253 Product name: Recombinant human RGPD5 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-230 aa of NM_001037866 Sequence: MRRSKADVERYVASVLGLTPSPRQKSMKGFYFAKLYYEAKEYDLAKKYICTYINVQERDPKAHRFLGLLYELEENTEKAVECYRRSVELNPTQKDLVLKIAELLCKNDVTDGRAKYWVERAAKLFPGSPAIYKLKEQLLDCEGEDGWNKLFDLIQSELYVRPDDVHVNIRLVELYRSTKRLKDAVAHCHEAERNIALRSSLEWNSCVVQTLKEYLESLQCLESDKSDWQA 相同性解析による交差性が予測される生物種 |
| Full Name | RANBP2-like and GRIP domain containing 5 |
| Calculated molecular weight | 198kDa |
| GenBank accession number | NM_001037866 |
| Gene Symbol | RGPD5 |
| Gene ID (NCBI) | 84220 |
| RRID | AB_3742459 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q99666 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The RGPD5 protein, full name RANBP2-like and GRIP domain containing 5, belongs to the RANBP2 (Ran-binding protein 2) gene family and is a member of this family generated through gene duplication. This protein is a large macromolecule whose functions are involved in the regulation of intracellular nucleocytoplasmic transport. It contains multiple functional domains, including the RanBD domain capable of binding to the small GTPase RAN, the GRIP domain that mediates protein-protein interactions, and regions associated with SUMO E3 ligase activity.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RGPD5 antibody 31636-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |